BMRB: 11313

Solution structure of the RING domain of the Zinc finger protein 183-like 1

Authors

Miyamoto, K., Sato, M., Tomizawa, T., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 183-like 1

Entity

1. RING domain (polymer, Thiol state: all other bound), 81 monomers, 8879.964 Da Detail

GSSGSSGGSE EEEIPFRCFI CRQAFQNPVV TKCRHYFCES CALEHFRATP RCYICDQPTG GIFNPAKELM AKLQKSGPSS G

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9010.782 Da

Max. entity weight

8879.964 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS38:SG	2:ZN1:ZN
4	na	sing	1:CYS41:SG	2:ZN1:ZN
5	na	sing	1:CYS33:SG	2:ZN1:ZN
6	na	sing	1:HIS35:ND1	2:ZN1:ZN
7	na	sing	1:CYS52:SG	2:ZN1:ZN
8	na	sing	1:CYS55:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.1 %, Completeness: 93.0 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.0 % (849 of 913)	92.9 % (446 of 480)	93.8 % (330 of 352)	90.1 % (73 of 81)
Backbone	89.7 % (425 of 474)	88.4 % (145 of 164)	90.6 % (213 of 235)	89.3 % (67 of 75)
Sidechain	96.1 % (492 of 512)	95.3 % (301 of 316)	97.4 % (185 of 190)	100.0 % (6 of 6)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. RING domain

GSSGSSGGSE EEEIPFRCFI CRQAFQNPVV TKCRHYFCES CALEHFRATP RCYICDQPTG GIFNPAKELM AKLQKSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.47mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 0.05mM {EDTA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.47 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	EDTA	natural abundance	salt	0.05 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CSY, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the Zinc finger protein 183-like 1
Miyamoto, K., Sato, M., Tomizawa, T., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 41 entities Detail

Interaction partners 1. RING domain, : 3 interactors Detail

Experiments performed 2 experiments Detail