BMRB: 11315

Solution structure of the RING domain of the Non-SMC element 1 protein

Authors

Miyamoto, K., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Non-SMC element 1 homolog

Entity

1. RING domain (polymer, Thiol state: all other bound), 74 monomers, 8037.973 Da Detail

GSSGSSGRET YPDAVKICNI CHSLLIQGQS CETCGIRMHL PCVAKYFQSN AEPRCPHCND YWPHEIPKSG PSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8168.791 Da

Max. entity weight

8037.973 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS39:ND1	2:ZN1:ZN
4	na	sing	1:CYS42:SG	2:ZN1:ZN
5	na	sing	1:CYS31:SG	2:ZN1:ZN
6	na	sing	1:CYS34:SG	2:ZN1:ZN
7	na	sing	1:CYS55:SG	2:ZN1:ZN
8	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 88.8 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.8 % (722 of 813)	87.1 % (371 of 426)	91.4 % (286 of 313)	87.8 % (65 of 74)
Backbone	86.7 % (373 of 430)	83.1 % (123 of 148)	88.8 % (191 of 215)	88.1 % (59 of 67)
Sidechain	91.1 % (410 of 450)	89.2 % (248 of 278)	94.5 % (156 of 165)	85.7 % (6 of 7)
Aromatic	93.5 % (58 of 62)	93.5 % (29 of 31)	93.3 % (28 of 30)	100.0 % (1 of 1)
Methyl	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (25 of 25)

1. RING domain

GSSGSSGRET YPDAVKICNI CHSLLIQGQS CETCGIRMHL PCVAKYFQSN AEPRCPHCND YWPHEIPKSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.27mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 0.1mM {NTA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.27 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	NTA	natural abundance	salt	0.1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT0, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the Non-SMC element 1 protein
Miyamoto, K., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 5 : 26 entities Detail

Interaction partners 1. RING domain, : 14 interactors Detail

Experiments performed 2 experiments Detail