BMRB: 11317

Solution Structure of the RING domain of the Tripartite motif protein 32

Authors

Miyamoto, K., Tochio, N., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Tripartite motif protein 32

Entity

1. RING domain (polymer, Thiol state: all other bound), 88 monomers, 9481.869 Da Detail

GSSGSSGNLD ALREVLECPI CMESFTEEQL RPKLLHCGHT ICRQCLEKLL ASSINGVRCP FCSKITRITS LTQLTDNLTV LKSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9612.6875 Da

Max. entity weight

9481.869 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS42:SG	2:ZN1:ZN
4	na	sing	1:CYS45:SG	2:ZN1:ZN
5	na	sing	1:CYS37:SG	2:ZN1:ZN
6	na	sing	1:HIS39:ND1	2:ZN1:ZN
7	na	sing	1:CYS59:SG	2:ZN1:ZN
8	na	sing	1:CYS62:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 91.8 %, Completeness (bb): 89.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (897 of 977)	90.4 % (459 of 508)	93.4 % (354 of 379)	93.3 % (84 of 90)
Backbone	89.8 % (467 of 520)	87.7 % (157 of 179)	90.3 % (232 of 257)	92.9 % (78 of 84)
Sidechain	94.2 % (507 of 538)	91.8 % (302 of 329)	98.0 % (199 of 203)	100.0 % (6 of 6)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)
Methyl	98.0 % (100 of 102)	96.1 % (49 of 51)	100.0 % (51 of 51)

1. RING domain

GSSGSSGNLD ALREVLECPI CMESFTEEQL RPKLLHCGHT ICRQCLEKLL ASSINGVRCP FCSKITRITS LTQLTDNLTV LKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.47mM RING domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 0.1mM {NTA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.47 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	NTA	natural abundance	salt	0.1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT2, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the RING domain of the Tripartite motif protein 32
Miyamoto, K., Tochio, N., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 80 entities Detail

Interaction partners 1. RING domain, : 37 interactors Detail

Experiments performed 2 experiments Detail