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Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4
Authors
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Assembly
Cdc42-interacting protein 4
Entity
1. Cdc42-interacting protein 4 (polymer, Thiol state: all free), 70 monomers, 7286.759 Da Detail

GSSGSSGGHC VAIYHFEGSS EGTISMAEGE DLSLMEEDKG DGWTRVRRKE GGEGYVPTSY LRVTSGPSSG


Formula weight
7286.759 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.6 %, Completeness: 90.9 %, Completeness (bb): 87.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.9 % (653 of 718)91.1 % (337 of 370)92.1 % (257 of 279)85.5 % (59 of 69)
Backbone87.3 % (363 of 416)86.2 % (131 of 152)88.8 % (174 of 196)85.3 % (58 of 68)
Sidechain95.3 % (341 of 358)94.5 % (206 of 218)96.4 % (134 of 139)100.0 % (1 of 1)
Aromatic100.0 % (54 of 54)100.0 % (27 of 27)100.0 % (26 of 26)100.0 % (1 of 1)
Methyl100.0 % (48 of 48)100.0 % (24 of 24)100.0 % (24 of 24)

1. SH3 domain

GSSGSSGGHC VAIYHFEGSS EGTISMAEGE DLSLMEEDKG DGWTRVRRKE GGEGYVPTSY LRVTSGPSSG

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.36mM SH3 domain {U-13C,15N;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90%H2O, 10%D2O


#NameIsotope labelingTypeConcentration
1SH3 domain[U-13C; U-15N]protein1.36 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.12 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.12 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.2 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2CT4, Strand ID: A Detail


Release date
2011-08-18
Citation
Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Related entities 1. Cdc42-interacting protein 4, : 1 : 3 : 109 entities Detail
Interaction partners 1. Cdc42-interacting protein 4, : 25 interactors Detail
Experiments performed 2 experiments Detail
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