BMRB: 11318

Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4

Authors

Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Cdc42-interacting protein 4

Entity

1. Cdc42-interacting protein 4 (polymer, Thiol state: all free), 70 monomers, 7286.759 Da Detail

GSSGSSGGHC VAIYHFEGSS EGTISMAEGE DLSLMEEDKG DGWTRVRRKE GGEGYVPTSY LRVTSGPSSG

Formula weight

7286.759 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 90.9 %, Completeness (bb): 87.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (653 of 718)	91.1 % (337 of 370)	92.1 % (257 of 279)	85.5 % (59 of 69)
Backbone	87.3 % (363 of 416)	86.2 % (131 of 152)	88.8 % (174 of 196)	85.3 % (58 of 68)
Sidechain	95.3 % (341 of 358)	94.5 % (206 of 218)	96.4 % (134 of 139)	100.0 % (1 of 1)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. SH3 domain

GSSGSSGGHC VAIYHFEGSS EGTISMAEGE DLSLMEEDKG DGWTRVRRKE GGEGYVPTSY LRVTSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.36mM SH3 domain {U-13C,15N;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90%H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.36 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT4, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Cdc42-interacting protein 4, : 1 : 3 : 109 entities Detail

Interaction partners 1. Cdc42-interacting protein 4, : 25 interactors Detail

Experiments performed 2 experiments Detail