BMRB: 11319

Solution Structure of the zinc finger BED domain of the zinc finger BED domain containing protein 1

Authors

Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger BED domain containing protein 1

Entity

1. zinc finger BED domain (polymer, Thiol state: free and other bound), 73 monomers, 8063.894 Da Detail

GSSGSSGSKV WKYFGFDTNA EGCILQWKKI YCRICMAQIA YSGNTSNLSY HLEKNHPEEF CEFVKSNSGP SSG

2. ZN (non-polymer), 65.409 Da

Total weight

8129.303 Da

Max. entity weight

8063.894 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS32:SG	2:ZN1:ZN
2	na	sing	1:CYS35:SG	2:ZN1:ZN
3	na	sing	1:HIS51:NE2	2:ZN1:ZN
4	na	sing	1:HIS56:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 89.1 %, Completeness (bb): 82.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.1 % (744 of 835)	89.2 % (387 of 434)	90.7 % (291 of 321)	82.5 % (66 of 80)
Backbone	82.9 % (360 of 434)	80.3 % (122 of 152)	85.8 % (181 of 211)	80.3 % (57 of 71)
Sidechain	95.1 % (443 of 466)	94.0 % (265 of 282)	96.6 % (169 of 175)	100.0 % (9 of 9)
Aromatic	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (50 of 50)	100.0 % (2 of 2)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. zinc finger BED domain

GSSGSSGSKV WKYFGFDTNA EGCILQWKKI YCRICMAQIA YSGNTSNLSY HLEKNHPEEF CEFVKSNSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.09mM zinc finger BED domain U-13C, {15N;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.01mM {ZNCl2;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zinc finger BED domain	[U-13C; U-15N]	protein	1.09 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.01 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT5, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the zinc finger BED domain of the zinc finger BED domain containing protein 1
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. zinc finger BED domain, : 1 : 11 entities Detail

Interaction partners 1. zinc finger BED domain, : 32 interactors Detail

Experiments performed 2 experiments Detail