BMRB: 11320

solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2

Authors

Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

sh3 domain-binding glutamic acid-rich-like protein 2

Entity

1. sh3 domain-binding glutamic acid-rich-like protein 2 (polymer, Thiol state: all free), 111 monomers, 12367.73 Da Detail

GSSGSSGMVI RVFIASSSGF VAIKKKQQDV VRFLEANKIE FEEVDITMSE EQRQWMYKNV PPEKKPTQGN PLPPQIFNGD RYCGDYDSFF ESKESNTVFS FLGLKSGPSS G

Formula weight

12367.73 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 96.2 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.2 % (1258 of 1308)	96.7 % (665 of 688)	96.0 % (484 of 504)	94.0 % (109 of 116)
Backbone	94.3 % (615 of 652)	93.8 % (211 of 225)	95.0 % (307 of 323)	93.3 % (97 of 104)
Sidechain	97.9 % (741 of 757)	98.1 % (454 of 463)	97.5 % (275 of 282)	100.0 % (12 of 12)
Aromatic	96.8 % (122 of 126)	100.0 % (63 of 63)	93.5 % (58 of 62)	100.0 % (1 of 1)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. SH3BGRL2

GSSGSSGMVI RVFIASSSGF VAIKKKQQDV VRFLEANKIE FEEVDITMSE EQRQWMYKNV PPEKKPTQGN PLPPQIFNGD RYCGDYDSFF ESKESNTVFS FLGLKSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.95mM SH3BGRL2 {U-13C,15N;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3BGRL2	[U-13C; U-15N]	protein	0.95 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT6, Strand ID: A Detail

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Release date

2011-08-18

Citation

solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2
Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. sh3 domain-binding glutamic acid-rich-like protein 2, : 1 : 3 : 48 entities Detail

Interaction partners 1. sh3 domain-binding glutamic acid-rich-like protein 2, : 2 interactors Detail

Experiments performed 2 experiments Detail