BMRB: 11321

Solution Structure of the IBR domain of the RING finger protein 31 protein

Authors

Miyamoto, K., Koshiba, S., Tomizawa, T., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

RING finger protein 31

Entity

1. IBR domain (polymer, Thiol state: all other bound), 86 monomers, 9937.114 Da Detail

GSSGSSGALF HKKLTEGVLM RDPKFLWCAQ CSFGFIYERE QLEATCPQCH QTFCVRCKRQ WEEQHRGRSC EDFQNWKRMN SGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

10067.933 Da

Max. entity weight

9937.114 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS28:SG	2:ZN1:ZN
2	na	sing	1:CYS31:SG	2:ZN1:ZN
3	na	sing	1:CYS46:SG	2:ZN1:ZN
4	na	sing	1:CYS49:SG	2:ZN1:ZN
5	na	sing	1:CYS54:SG	2:ZN1:ZN
6	na	sing	1:CYS57:SG	2:ZN1:ZN
7	na	sing	1:HIS65:NE2	2:ZN1:ZN
8	na	sing	1:CYS70:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 91.6 %, Completeness (bb): 87.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (930 of 1015)	92.2 % (495 of 537)	92.7 % (355 of 383)	84.2 % (80 of 95)
Backbone	87.5 % (446 of 510)	86.4 % (153 of 177)	90.0 % (225 of 250)	81.9 % (68 of 83)
Sidechain	95.7 % (558 of 583)	95.0 % (342 of 360)	96.7 % (204 of 211)	100.0 % (12 of 12)
Aromatic	94.8 % (110 of 116)	94.8 % (55 of 58)	94.5 % (52 of 55)	100.0 % (3 of 3)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. IBR domain

GSSGSSGALF HKKLTEGVLM RDPKFLWCAQ CSFGFIYERE QLEATCPQCH QTFCVRCKRQ WEEQHRGRSC EDFQNWKRMN SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.55mM IBR domain {U-13C,15N;} 20mM {d-Tris-HCl;} 200mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.01mM {ZnCl2;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	IBR domain	[U-13C; U-15N]	protein	1.55 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	200 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.01 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.37 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CT7, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the IBR domain of the RING finger protein 31 protein
Miyamoto, K., Koshiba, S., Tomizawa, T., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. IBR domain, : 1 : 11 : 12 entities Detail

Interaction partners 1. IBR domain, : 32 interactors Detail

Experiments performed 2 experiments Detail