BMRB: 11322

Solution structure of the SANT domain of human KIAA1915 protein

Authors

Yoneyama, M., Umehara, T., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Tanaka, A., Kigawa, T., Yokoyama, S.

Assembly

KIAA1915 protein

Entity

1. KIAA1915 protein (polymer, Thiol state: all free), 72 monomers, 8160.238 Da Detail

GSSGSSGYSV KWTIEEKELF EQGLAKFGRR WTKISKLIGS RTVLQVKSYA RQYFKNKVKC GLDKETPNQK TG

Formula weight

8160.238 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 97.5 %, Completeness (bb): 97.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.5 % (855 of 877)	96.8 % (449 of 464)	98.8 % (330 of 334)	96.2 % (76 of 79)
Backbone	97.2 % (418 of 430)	95.4 % (144 of 151)	98.1 % (204 of 208)	98.6 % (70 of 71)
Sidechain	98.0 % (501 of 511)	97.4 % (305 of 313)	100.0 % (190 of 190)	75.0 % (6 of 8)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	98.4 % (61 of 62)	96.8 % (30 of 31)	100.0 % (31 of 31)

1. SANT domain

GSSGSSGYSV KWTIEEKELF EQGLAKFGRR WTKISKLIGS RTVLQVKSYA RQYFKNKVKC GLDKETPNQK TG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 4.5 (±0.05), Details 0.83mM SANT domain U-15N, {13C;} 20mM Acetate buffer Na {(pH4.5);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SANT domain	[U-13C; U-15N]	protein	0.83 mM
2	Acetate buffer Na	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.63 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2CU7, Strand ID: A Detail

---

Release date

2010-11-30

Citation

Structural and functional differences of SWIRM domain subtypes
Yoneyama, M., Tochio, N., Umehara, T., Koshiba, S., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Watanabe, S., Tomo, Y., Nishimura, Y., Harada, T., Terada, T., Shirouzu, M., Hayashizaki, Y., Ohara, O., Tanaka, A., Kigawa, T., Yokoyama, S.
J. Mol. Biol. (2007), 369, 222-238, PubMed 17428495 , DOI 10.1016/j.jmb.2007.03.027 , Abstract

Related entities 1. KIAA1915 protein, : 1 : 57 entities Detail

Experiments performed 2 experiments Detail