BMRB: 11323

Solution structure of the MYND domain of the human zinc finger MYND domain-containing protein 10

Authors

Miyamoto, K., Kigawa, T., Tomizawa, T., Tochio, N., Sasagawa, A., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Zinc finger MYND domain containing protein 10

Entity

1. zf-MYND (polymer, Thiol state: free and other bound), 70 monomers, 7537.350 Da Detail

GSSGSSGLEA VAPERPRCAY CSAEASKRCS RCQNEWYCCR ECQVKHWEKH GKTCVLAAQG DRAKSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7668.168 Da

Max. entity weight

7537.35 Da

Entity Connection

na 8 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS38:SG	2:ZN1:ZN
4	na	sing	1:CYS42:SG	2:ZN1:ZN
5	na	sing	1:CYS29:SG	2:ZN1:ZN
6	na	sing	1:CYS32:SG	2:ZN1:ZN
7	na	sing	1:HIS50:NE2	2:ZN1:ZN
8	na	sing	1:CYS54:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 91.3 %, Completeness (bb): 89.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (681 of 746)	90.1 % (354 of 393)	92.9 % (260 of 280)	91.8 % (67 of 73)
Backbone	89.9 % (372 of 414)	88.2 % (127 of 144)	90.6 % (184 of 203)	91.0 % (61 of 67)
Sidechain	92.9 % (367 of 395)	91.2 % (227 of 249)	95.7 % (134 of 140)	100.0 % (6 of 6)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (22 of 22)	100.0 % (2 of 2)
Methyl	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)

1. zf-MYND

GSSGSSGLEA VAPERPRCAY CSAEASKRCS RCQNEWYCCR ECQVKHWEKH GKTCVLAAQG DRAKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10%D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.3mM zf-MYND {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.1mM {ZNCl2;} 90% H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	zf-MYND	[U-13C; U-15N]	protein	0.3 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.1 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2D8Q, Strand ID: A Detail

---

Release date

2011-08-18

Citation

Solution structure of the MYND domain of the human zinc finger MYND domain-containing protein 10
Miyamoto, K., Kigawa, T., Tomizawa, T., Tochio, N., Sasagawa, A., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. zf-MYND, : 1 : 2 : 23 entities Detail

Experiments performed 2 experiments Detail