BMRB: 11325

Solution structure of the RING domain of the human cellular modulator of immune recognition protein

Authors

Miyamoto, K., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

cellular modulator of immune recognition

Entity

1. RING domain (polymer, Thiol state: free and other bound), 80 monomers, 8613.577 Da Detail

GSSGSSGTSI TPSSQDICRI CHCEGDDESP LITPCHCTGS LHFVHQACLQ QWIKSSDTRC CELCKYEFIM ETKLSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8744.3955 Da

Max. entity weight

8613.577 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS45:ND1	2:ZN1:ZN
4	na	sing	1:CYS48:SG	2:ZN1:ZN
5	na	sing	1:CYS35:SG	2:ZN1:ZN
6	na	sing	1:CYS37:SG	2:ZN1:ZN
7	na	sing	1:CYS61:SG	2:ZN1:ZN
8	na	sing	1:CYS64:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 89.8 %, Completeness (bb): 87.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (770 of 857)	89.6 % (397 of 443)	91.0 % (303 of 333)	86.4 % (70 of 81)
Backbone	87.9 % (415 of 472)	86.5 % (141 of 163)	89.3 % (208 of 233)	86.8 % (66 of 76)
Sidechain	91.9 % (421 of 458)	91.4 % (256 of 280)	93.1 % (161 of 173)	80.0 % (4 of 5)
Aromatic	91.1 % (51 of 56)	92.9 % (26 of 28)	88.9 % (24 of 27)	100.0 % (1 of 1)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. RING domain

GSSGSSGTSI TPSSQDICRI CHCEGDDESP LITPCHCTGS LHFVHQACLQ QWIKSSDTRC CELCKYEFIM ETKLSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.22mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.0 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D8S, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the human cellular modulator of immune recognition protein
Miyamoto, K., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. RING domain, : 1 : 2 : 45 entities Detail

Interaction partners 1. RING domain, : 1 interactors Detail

Experiments performed 2 experiments Detail