BMRB: 11326

Solution structure of the RING domain of the human RING finger protein 146

Authors

Kigawa, T., Miyamoto, K., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

RING finger protein 146

Entity

1. RING domain (polymer, Thiol state: free and other bound), 71 monomers, 7400.452 Da Detail

GSSGSSGNTA PSLTVPECAI CLQTCVHPVS LPCKHVFCYL CVKGASWLGK RCALCRQEIP EDFLDSGPSS G

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7531.27 Da

Max. entity weight

7400.452 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS38:SG	2:ZN1:ZN
4	na	sing	1:CYS41:SG	2:ZN1:ZN
5	na	sing	1:CYS33:SG	2:ZN1:ZN
6	na	sing	1:HIS35:ND1	2:ZN1:ZN
7	na	sing	1:CYS52:SG	2:ZN1:ZN
8	na	sing	1:CYS55:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.1 %, Completeness: 90.8 %, Completeness (bb): 86.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (689 of 759)	91.6 % (358 of 391)	91.0 % (272 of 299)	85.5 % (59 of 69)
Backbone	86.5 % (358 of 414)	86.7 % (124 of 143)	86.9 % (179 of 206)	84.6 % (55 of 65)
Sidechain	95.1 % (389 of 409)	94.4 % (234 of 248)	96.2 % (151 of 157)	100.0 % (4 of 4)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (23 of 23)	100.0 % (1 of 1)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. RING domain

GSSGSSGNTA PSLTVPECAI CLQTCVHPVS LPCKHVFCYL CVKGASWLGK RCALCRQEIP EDFLDSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.34mM RING domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.34 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D8T, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the human RING finger protein 146
Miyamoto, K., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. RING domain, : 1 : 11 : 42 entities Detail

Interaction partners 1. RING domain, : 10 interactors Detail

Experiments performed 2 experiments Detail