BMRB: 11329

Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2

Authors

Yoneyama, M., Kigawa, T., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Possible global transcription activator SNF2L2

Entity

1. Possible global transcription activator SNF2L2 (polymer, Thiol state: all free), 123 monomers, 13861.56 Da Detail

GSSGSSGSPN PPKLTKQMNA IIDTVINYKD SSGRQLSEVF IQLPSRKELP EYYELIRKPV DFKKIKERIR NHKYRSLGDL EKDVMLLCHN AQTFNLEGSQ IYEDSIVLQS VFKSARQSGP SSG

Formula weight

13861.56 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 92.1 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (1348 of 1464)	90.1 % (698 of 775)	95.2 % (533 of 560)	90.7 % (117 of 129)
Backbone	90.3 % (654 of 724)	87.0 % (215 of 247)	92.5 % (334 of 361)	90.5 % (105 of 116)
Sidechain	94.0 % (804 of 855)	91.5 % (483 of 528)	98.4 % (309 of 314)	92.3 % (12 of 13)
Aromatic	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)
Methyl	99.1 % (115 of 116)	98.3 % (57 of 58)	100.0 % (58 of 58)

1. Bromodomain

GSSGSSGSPN PPKLTKQMNA IIDTVINYKD SSGRQLSEVF IQLPSRKELP EYYELIRKPV DFKKIKERIR NHKYRSLGDL EKDVMLLCHN AQTFNLEGSQ IYEDSIVLQS VFKSARQSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.39mM Bromodomain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 75mM Guanidine {oxalate;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Bromodomain	[U-13C; U-15N]	protein	1.39 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	Guanidine oxalate	natural abundance	salt	75 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAT, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2
Yoneyama, M., Kigawa, T., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Possible global transcription activator SNF2L2, : 1 : 12 : 155 entities Detail

Interaction partners 1. Possible global transcription activator SNF2L2, : 1 interactors Detail

Experiments performed 2 experiments Detail