BMRB: 11332

Solution structure of the RING domain of the human Polycomb group RING finger protein 6

Authors

Miyamoto, K., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Polycomb group RING finger protein 6

Entity

1. RING domain (polymer, Thiol state: all other bound), 72 monomers, 7788.850 Da Detail

GSSGSSGNLS ELTPYILCSI CKGYLIDATT ITECLHTFCK SCIVRHFYYS NRCPKCNIVV HQTQPLSGPS SG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7919.668 Da

Max. entity weight

7788.85 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS39:SG	2:ZN1:ZN
4	na	sing	1:CYS42:SG	2:ZN1:ZN
5	na	sing	1:CYS34:SG	2:ZN1:ZN
6	na	sing	1:HIS36:ND1	2:ZN1:ZN
7	na	sing	1:CYS53:SG	2:ZN1:ZN
8	na	sing	1:CYS56:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 95.2 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (760 of 798)	95.1 % (390 of 410)	95.9 % (302 of 315)	93.2 % (68 of 73)
Backbone	93.9 % (398 of 424)	93.2 % (136 of 146)	94.8 % (199 of 210)	92.6 % (63 of 68)
Sidechain	96.6 % (425 of 440)	96.2 % (254 of 264)	97.1 % (166 of 171)	100.0 % (5 of 5)
Aromatic	93.8 % (60 of 64)	93.8 % (30 of 32)	93.8 % (30 of 32)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. RING domain

GSSGSSGNLS ELTPYILCSI CKGYLIDATT ITECLHTFCK SCIVRHFYYS NRCPKCNIVV HQTQPLSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.43mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	0.43 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.34 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJB, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the human Polycomb group RING finger protein 6
Miyamoto, K., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. RING domain, : 1 : 6 : 79 entities Detail

Interaction partners 1. RING domain, : 39 interactors Detail

Experiments performed 2 experiments Detail