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BMRB: 11333


11333
2DKT
Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus
Authors
Miyamoto, K., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
Assembly
RING finger and CHY zinc finger domain-containing protein 1
Entity
1. CHY domian (polymer, Thiol state: free and other bound), 143 monomers, 16120.20 Da Detail

GSSGSSGGVR NLAQGPRGCE HYDRACLLKA PCCDKLYTCR LCHDTNEDHQ LDRFKVKEVQ CINCEKLQHA QQTCEDCSTL FGEYYCSICH LFDKDKRQYH CESCGICRIG PKEDFFHCLK CNLCLTTNLR GKHKCIESGP SSG


2. ZN (non-polymer), 65.409 × 6 Da
Total weight
16512.654 Da
Max. entity weight
16120.2 Da
Entity Connection
na 24 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS19:SG2:ZN1:ZN
2nasing1:HIS21:ND12:ZN1:ZN
3nasing1:CYS39:SG2:ZN1:ZN
4nasing1:CYS42:SG2:ZN1:ZN
5nasing1:CYS32:SG2:ZN1:ZN
6nasing1:CYS33:SG2:ZN1:ZN
7nasing1:HIS43:NE22:ZN1:ZN
8nasing1:HIS49:NE22:ZN1:ZN
9nasing1:CYS61:SG2:ZN1:ZN
10nasing1:CYS64:SG2:ZN1:ZN
11nasing1:CYS74:SG2:ZN1:ZN
12nasing1:CYS77:SG2:ZN1:ZN
13nasing1:CYS86:SG2:ZN1:ZN
14nasing1:CYS89:SG2:ZN1:ZN
15nasing1:HIS100:ND12:ZN1:ZN
16nasing1:CYS107:SG2:ZN1:ZN
17nasing1:CYS101:SG2:ZN1:ZN
18nasing1:CYS104:SG2:ZN1:ZN
19nasing1:HIS117:ND12:ZN1:ZN
20nasing1:CYS124:SG2:ZN1:ZN
21nasing1:CYS118:SG2:ZN1:ZN
22nasing1:CYS121:SG2:ZN1:ZN
23nasing1:HIS133:NE22:ZN1:ZN
24nasing1:CYS135:SG2:ZN1:ZN

Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.4 %, Completeness: 92.0 %, Completeness (bb): 90.4 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All92.0 % (1486 of 1616)91.7 % (778 of 848)92.9 % (573 of 617)89.4 % (135 of 151)
Backbone90.4 % (768 of 850)88.8 % (261 of 294)92.1 % (384 of 417)88.5 % (123 of 139)
Sidechain94.0 % (843 of 897)93.3 % (517 of 554)94.9 % (314 of 331)100.0 % (12 of 12)
Aromatic85.2 % (104 of 122)86.9 % (53 of 61)83.6 % (51 of 61)
Methyl97.1 % (101 of 104)96.2 % (50 of 52)98.1 % (51 of 52)

1. CHY domian

GSSGSSGGVR NLAQGPRGCE HYDRACLLKA PCCDKLYTCR LCHDTNEDHQ LDRFKVKEVQ CINCEKLQHA QQTCEDCSTL FGEYYCSICH LFDKDKRQYH CESCGICRIG PKEDFFHCLK CNLCLTTNLR GKHKCIESGP SSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.23mM CHY domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.1mM {ZNCl2;} 90%H2O, 10%D2O


#NameIsotope labelingTypeConcentration
1CHY domain[U-13C; U-15N]protein1.23 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6ZnCl2natural abundancesalt0.1 mM
7H2Osolvent90 %
8D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.62 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2DKT, Strand ID: A Detail

Release date
2011-08-18
Citation
Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus
Miyamoto, K., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
Related entities 1. CHY domian, : 1 : 58 entities Detail
More details for 59 related entities
Experiments performed 2 experiments Detail
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