BMRB: 11334

Solution structure of the TFIIS domain II of human PHD finger protein 3

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

PHD finger protein 3

Entity

1. PHD finger protein 3 (polymer, Thiol state: not present), 120 monomers, 13582.44 Da Detail

GSSGSSGSAD QIRQSVRHSL KDILMKRLTD SNLKVPEEKA AKVATKIEKE LFSFFRDTDA KYKNKYRSLM FNLKDPKNNI LFKKVLKGEV TPDHLIRMSP EELASKELAA WRRRSGPSSG

Formula weight

13582.44 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 91.5 %, Completeness (bb): 88.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.5 % (1318 of 1440)	90.8 % (694 of 764)	93.1 % (515 of 553)	88.6 % (109 of 123)
Backbone	88.3 % (627 of 710)	85.5 % (206 of 241)	90.4 % (320 of 354)	87.8 % (101 of 115)
Sidechain	94.4 % (797 of 844)	93.3 % (488 of 523)	96.2 % (301 of 313)	100.0 % (8 of 8)
Aromatic	97.7 % (84 of 86)	97.7 % (42 of 43)	97.6 % (41 of 42)	100.0 % (1 of 1)
Methyl	96.4 % (108 of 112)	96.4 % (54 of 56)	96.4 % (54 of 56)

1. TFSII M domain

GSSGSSGSAD QIRQSVRHSL KDILMKRLTD SNLKVPEEKA AKVATKIEKE LFSFFRDTDA KYKNKYRSLM FNLKDPKNNI LFKKVLKGEV TPDHLIRMSP EELASKELAA WRRRSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.20mM TFSII_M domain U-15N, {13C;} 20mM d-Tris HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	TFSII_M domain	[U-13C; U-15N]	protein	1.20 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DME, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the TFIIS domain II of human PHD finger protein 3
Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. PHD finger protein 3, : 1 : 64 entities Detail

Interaction partners 1. PHD finger protein 3, : 8 interactors Detail

Experiments performed 2 experiments Detail