BMRB: 11336

Solution Structure of the RING domain of the human ring finger protein 4

Authors

Miyamoto, K., Yoneyama, M., Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RING finger protein 4

Entity

1. RING domain (polymer, Thiol state: all other bound), 69 monomers, 7291.253 Da Detail

GSSGSSGTGL RPSGTVSCPI CMDGYSEIVQ NGRLIVSTEC GHVFCSQCLR DSLKNANTCP TCRKKINHK

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7422.071 Da

Max. entity weight

7291.253 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS45:SG	2:ZN1:ZN
4	na	sing	1:CYS48:SG	2:ZN1:ZN
5	na	sing	1:CYS40:SG	2:ZN1:ZN
6	na	sing	1:HIS42:ND1	2:ZN1:ZN
7	na	sing	1:CYS59:SG	2:ZN1:ZN
8	na	sing	1:CYS62:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.8 %, Completeness: 93.8 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.8 % (691 of 737)	93.8 % (360 of 384)	95.0 % (267 of 281)	88.9 % (64 of 72)
Backbone	91.4 % (373 of 408)	90.9 % (130 of 143)	93.0 % (185 of 199)	87.9 % (58 of 66)
Sidechain	96.2 % (375 of 390)	95.4 % (230 of 241)	97.2 % (139 of 143)	100.0 % (6 of 6)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. RING domain

GSSGSSGTGL RPSGTVSCPI CMDGYSEIVQ NGRLIVSTEC GHVFCSQCLR DSLKNANTCP TCRKKINHK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.03mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.03 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EA6, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the RING domain of the human ring finger protein 4
Miyamoto, K., Yoneyama, M., Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 14 : 80 entities Detail

Interaction partners 1. RING domain, : 21 interactors Detail

Experiments performed 2 experiments Detail