BMRB: 11337

Solution structure of the RWD domain of human RWD domain containing protein 3

Authors

Yoneyama, M., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RWD domain-containing protein 3

Entity

1. RWD domain-containing protein 3 (polymer, Thiol state: all free), 128 monomers, 14224.03 Da Detail

GSSGSSGMAE PVQEELSVLA AIFCRPHEWE VLSRSETDGT VFRIHTKAEG FMDADIPLEL VFHLPVNYPS CLPGISINSE QLTRAQCVTV KEKLLEQAES LLSEPMVHEL VLWIQQNLRH ILSQPETG

Formula weight

14224.03 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 95.5 %, Completeness (bb): 94.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (1407 of 1474)	95.2 % (726 of 763)	95.7 % (554 of 579)	96.2 % (127 of 132)
Backbone	94.0 % (707 of 752)	93.3 % (238 of 255)	93.9 % (354 of 377)	95.8 % (115 of 120)
Sidechain	96.8 % (816 of 843)	96.1 % (488 of 508)	97.8 % (316 of 323)	100.0 % (12 of 12)
Aromatic	95.7 % (88 of 92)	95.7 % (44 of 46)	95.5 % (42 of 44)	100.0 % (2 of 2)
Methyl	98.7 % (156 of 158)	97.5 % (77 of 79)	100.0 % (79 of 79)

1. RWD domain

GSSGSSGMAE PVQEELSVLA AIFCRPHEWE VLSRSETDGT VFRIHTKAEG FMDADIPLEL VFHLPVNYPS CLPGISINSE QLTRAQCVTV KEKLLEQAES LLSEPMVHEL VLWIQQNLRH ILSQPETG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.63mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	0.63 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.09 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EBK, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RWD domain of human RWD domain containing protein 3
Yoneyama, M., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RWD domain-containing protein 3, : 1 : 2 : 19 entities Detail

Interaction partners 1. RWD domain-containing protein 3, : 4 interactors Detail

Experiments performed 2 experiments Detail