BMRB: 11338

Solution structure of the RWD domain of human RWD domain containing protein 1

Authors

Yoneyama, M., Tomizawa, T., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RWD domain-containing protein 1

Entity

1. RWD domain-containing protein 1 (polymer, Thiol state: not present), 128 monomers, 14297.54 Da Detail

GSSGSSGMTD YGEEQRNELE ALESIYPDSF TVLSENPPSF TITVTSEAGE NDETVQTTLK FTYSEKYPDE APLYEIFSQE NLEDNDVSDI LKLLALQAEE NLGMVMIFTL VTAVQEKLNE IVDQIKTR

Formula weight

14297.54 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 96.1 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1400 of 1457)	95.7 % (719 of 751)	96.7 % (551 of 570)	95.6 % (130 of 136)
Backbone	95.1 % (721 of 758)	93.4 % (240 of 257)	96.0 % (363 of 378)	95.9 % (118 of 123)
Sidechain	97.3 % (799 of 821)	97.0 % (479 of 494)	98.1 % (308 of 314)	92.3 % (12 of 13)
Aromatic	95.6 % (86 of 90)	97.8 % (44 of 45)	93.3 % (42 of 45)
Methyl	99.3 % (147 of 148)	98.6 % (73 of 74)	100.0 % (74 of 74)

1. RWD domain

GSSGSSGMTD YGEEQRNELE ALESIYPDSF TVLSENPPSF TITVTSEAGE NDETVQTTLK FTYSEKYPDE APLYEIFSQE NLEDNDVSDI LKLLALQAEE NLGMVMIFTL VTAVQEKLNE IVDQIKTR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.19mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	1.19 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.28 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.28 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EBM, Strand ID: A Detail

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Release date

2011-08-18

Citation

RWD Domain as an E2 (Ubc9)-Interaction Module
Yoneyama, M., Tomizawa, T., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.
J. Biol. Chem. (2015), 290, 16550-16559, PubMed 25918163 , DOI 10.1074/jbc.M115.644047 , Abstract

Related entities 1. RWD domain-containing protein 1, : 1 : 4 : 75 entities Detail

Interaction partners 1. RWD domain-containing protein 1, : 6 interactors Detail

Experiments performed 2 experiments Detail