Solution structure of the RWD domain of human RWD domain containing protein 1
GSSGSSGMTD YGEEQRNELE ALESIYPDSF TVLSENPPSF TITVTSEAGE NDETVQTTLK FTYSEKYPDE APLYEIFSQE NLEDNDVSDI LKLLALQAEE NLGMVMIFTL VTAVQEKLNE IVDQIKTR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.1 % (1400 of 1457) | 95.7 % (719 of 751) | 96.7 % (551 of 570) | 95.6 % (130 of 136) |
Backbone | 95.1 % (721 of 758) | 93.4 % (240 of 257) | 96.0 % (363 of 378) | 95.9 % (118 of 123) |
Sidechain | 97.3 % (799 of 821) | 97.0 % (479 of 494) | 98.1 % (308 of 314) | 92.3 % (12 of 13) |
Aromatic | 95.6 % (86 of 90) | 97.8 % (44 of 45) | 93.3 % (42 of 45) | |
Methyl | 99.3 % (147 of 148) | 98.6 % (73 of 74) | 100.0 % (74 of 74) |
1. RWD domain
GSSGSSGMTD YGEEQRNELE ALESIYPDSF TVLSENPPSF TITVTSEAGE NDETVQTTLK FTYSEKYPDE APLYEIFSQE NLEDNDVSDI LKLLALQAEE NLGMVMIFTL VTAVQEKLNE IVDQIKTRSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.19mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RWD domain | [U-13C; U-15N] | protein | 1.19 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |