BMRB: 11339

Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens

Authors

Miyamoto, K., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Baculoviral IAP repeat-containing protein 4

Entity

1. RING domain (polymer, Thiol state: all other bound), 75 monomers, 8390.816 Da Detail

GSSGSSGSLQ KEISTEEQLR RLQEEKLCKI CMDRNIAIVF VPCGHLVTCK QCAEAVDKCP MCYTVITFKQ KIFMS

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8521.635 Da

Max. entity weight

8390.816 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS28:SG	2:ZN1:ZN
2	na	sing	1:CYS31:SG	2:ZN1:ZN
3	na	sing	1:CYS43:SG	2:ZN1:ZN
4	na	sing	1:HIS45:ND1	2:ZN1:ZN
5	na	sing	1:CYS49:SG	2:ZN1:ZN
6	na	sing	1:CYS52:SG	2:ZN1:ZN
7	na	sing	1:CYS59:SG	2:ZN1:ZN
8	na	sing	1:CYS62:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 94.4 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (825 of 874)	94.3 % (432 of 458)	95.3 % (321 of 337)	91.1 % (72 of 79)
Backbone	91.0 % (406 of 446)	88.8 % (135 of 152)	92.8 % (205 of 221)	90.4 % (66 of 73)
Sidechain	97.4 % (486 of 499)	97.1 % (297 of 306)	97.9 % (183 of 187)	100.0 % (6 of 6)
Aromatic	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. RING domain

GSSGSSGSLQ KEISTEEQLR RLQEEKLCKI CMDRNIAIVF VPCGHLVTCK QCAEAVDKCP MCYTVITFKQ KIFMS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM RING domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECG, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens
Miyamoto, K., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 5 : 108 entities Detail

Interaction partners 1. RING domain, : 6 interactors Detail

Experiments performed 2 experiments Detail