BMRB: 11340

Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein

Authors

Miyamoto, K., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

TNF receptor-associated factor 6

Entity

1. RING domain (polymer, Thiol state: all other bound), 86 monomers, 9603.899 Da Detail

GSSGSSGMEE IQGYDVEFDP PLESKYECPI CLMALREAVQ TPCGHRFCKA CIIKSIRDAG HKCPVDNEIL LENQLFPDNF AKREIL

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9734.718 Da

Max. entity weight

9603.899 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS28:SG	2:ZN1:ZN
2	na	sing	1:CYS31:SG	2:ZN1:ZN
3	na	sing	1:CYS43:SG	2:ZN1:ZN
4	na	sing	1:HIS45:ND1	2:ZN1:ZN
5	na	sing	1:CYS48:SG	2:ZN1:ZN
6	na	sing	1:CYS51:SG	2:ZN1:ZN
7	na	sing	1:CYS63:SG	2:ZN1:ZN
8	na	sing	1:ASP66:OD2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 96.4 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (954 of 990)	96.2 % (500 of 520)	97.4 % (374 of 384)	93.0 % (80 of 86)
Backbone	94.4 % (476 of 504)	93.0 % (160 of 172)	96.0 % (242 of 252)	92.5 % (74 of 80)
Sidechain	98.2 % (556 of 566)	97.7 % (340 of 348)	99.1 % (210 of 212)	100.0 % (6 of 6)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. RING domain

GSSGSSGMEE IQGYDVEFDP PLESKYECPI CLMALREAVQ TPCGHRFCKA CIIKSIRDAG HKCPVDNEIL LENQLFPDNF AKREIL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.08mM RING domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.08 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECI, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein
Miyamoto, K., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 6 : 61 entities Detail

Interaction partners 1. RING domain, : 384 interactors Detail

Experiments performed 2 experiments Detail