Solution structure of the RING domain of the human tripartite motif-containing protein 39
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS21:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS38:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS41:SG | 2:ZN1:ZN |
5 | na | sing | 1:CYS33:SG | 2:ZN1:ZN |
6 | na | sing | 1:HIS35:ND1 | 2:ZN1:ZN |
7 | na | sing | 1:CYS55:SG | 2:ZN1:ZN |
8 | na | sing | 1:CYS58:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.0 % (595 of 647) | 91.6 % (306 of 334) | 92.5 % (234 of 253) | 91.7 % (55 of 60) |
Backbone | 88.6 % (303 of 342) | 87.2 % (102 of 117) | 88.8 % (151 of 170) | 90.9 % (50 of 55) |
Sidechain | 95.3 % (342 of 359) | 94.0 % (204 of 217) | 97.1 % (133 of 137) | 100.0 % (5 of 5) |
Aromatic | 100.0 % (56 of 56) | 100.0 % (28 of 28) | 100.0 % (26 of 26) | 100.0 % (2 of 2) |
Methyl | 96.4 % (54 of 56) | 92.9 % (26 of 28) | 100.0 % (28 of 28) |
1. RING domain
GSSGSSGALE NLQVEASCSV CLEYLKEPVI IECGHNFCKA CITRWWEDLE RDFPCPVCSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.06mM RING domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RING domain | [U-13C; U-15N] | protein | 1.06 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | ZnCl2 | natural abundance | salt | 0.05 mM |
7 | IDA | natural abundance | salt | 1.0 mM |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |