BMRB: 11343

Solution structure of the RING domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus

Authors

Miyamoto, K., Yoneyama, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RING finger and CHY zinc finger domain-containing protein 1

Entity

1. RING domain (polymer, Thiol state: all other bound), 55 monomers, 5831.623 Da Detail

GSSGSSGCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCS GPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

5962.441 Da

Max. entity weight

5831.623 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS32:ND1	2:ZN1:ZN
4	na	sing	1:CYS35:SG	2:ZN1:ZN
5	na	sing	1:CYS27:SG	2:ZN1:ZN
6	na	sing	1:HIS29:ND1	2:ZN1:ZN
7	na	sing	1:CYS46:SG	2:ZN1:ZN
8	na	sing	1:CYS49:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 94.0 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.0 % (544 of 579)	93.0 % (278 of 299)	95.6 % (219 of 229)	92.2 % (47 of 51)
Backbone	92.9 % (299 of 322)	92.0 % (104 of 113)	93.7 % (148 of 158)	92.2 % (47 of 51)
Sidechain	95.4 % (291 of 305)	93.5 % (174 of 186)	98.3 % (117 of 119)
Aromatic	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	96.0 % (48 of 50)	92.0 % (23 of 25)	100.0 % (25 of 25)

1. RING domain

GSSGSSGCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECM, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RING domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus
Miyamoto, K., Yoneyama, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RING domain, : 1 : 172 entities Detail

Experiments performed 2 experiments Detail