BMRB: 11344

Solution structure of the RING domain of the human RING finger protein 141

Authors

Miyamoto, K., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RING finger protein 141

Entity

1. RING domain (polymer, Thiol state: free and other bound), 70 monomers, 7564.455 Da Detail

GSSGSSGRVK QLTDEEECCI CMDGRADLIL PCAHSFCQKC IDKWSDRHRN CPICRLQMTG ANESSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7695.273 Da

Max. entity weight

7564.455 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS37:SG	2:ZN1:ZN
4	na	sing	1:CYS40:SG	2:ZN1:ZN
5	na	sing	1:CYS32:SG	2:ZN1:ZN
6	na	sing	1:HIS34:ND1	2:ZN1:ZN
7	na	sing	1:CYS51:SG	2:ZN1:ZN
8	na	sing	1:CYS54:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 90.9 %, Completeness (bb): 87.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (678 of 746)	90.3 % (354 of 392)	92.9 % (261 of 281)	86.3 % (63 of 73)
Backbone	87.4 % (362 of 414)	84.7 % (122 of 144)	90.1 % (183 of 203)	85.1 % (57 of 67)
Sidechain	94.7 % (374 of 395)	93.5 % (232 of 248)	96.5 % (136 of 141)	100.0 % (6 of 6)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (14 of 14)	100.0 % (1 of 1)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. RING domain

GSSGSSGRVK QLTDEEECCI CMDGRADLIL PCAHSFCQKC IDKWSDRHRN CPICRLQMTG ANESSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.31mM RING domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZNCl2;} 1.0mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RING domain	[U-13C; U-15N]	protein	1.31 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1.0 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECN, Strand ID: A Detail

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Release date

2011-08-18

Citation

The zinc finger domain of RING finger protein 141 reveals a unique RING fold
Miyamoto, K., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Protein Sci. (2017), 26, 1681-1686, PubMed 28547869 , DOI 10.1002/pro.3201 , Abstract

Related entities 1. RING domain, : 1 : 8 : 48 entities Detail

Interaction partners 1. RING domain, : 4 interactors Detail

Experiments performed 2 experiments Detail