BMRB: 11345

Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Tomizawa, T., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.

Assembly

Transcriptional adapter 2

Entity

1. Transcriptional adapter 2 (polymer, Thiol state: all free), 88 monomers, 9947.342 Da Detail

GSSGSSGNMT ISDIQHAPDY ALLSNDEQQL CIQLKILPKP YLVLKEVMFR ELLKTGGNLS KSACRELLNI DPIKANRIYD FFQSQNWM

Formula weight

9947.342 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 94.9 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (1000 of 1054)	95.1 % (526 of 553)	95.3 % (385 of 404)	91.8 % (89 of 97)
Backbone	92.3 % (480 of 520)	92.1 % (163 of 177)	92.7 % (240 of 259)	91.7 % (77 of 84)
Sidechain	97.2 % (600 of 617)	96.5 % (363 of 376)	98.7 % (225 of 228)	92.3 % (12 of 13)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (34 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. SWIRM

GSSGSSGNMT ISDIQHAPDY ALLSNDEQQL CIQLKILPKP YLVLKEVMFR ELLKTGGNLS KSACRELLNI DPIKANRIYD FFQSQNWM

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.3mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SWIRM	[U-13C; U-15N]	protein	0.3 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.58 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.58 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.27 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ELJ, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2
Yoneyama, M., Tochio, N., Koshiba, S., Tomizawa, T., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.

Related entities 1. Transcriptional adapter 2, : 1 : 2 : 29 entities Detail

Interaction partners 1. Transcriptional adapter 2, : 101 interactors Detail

Experiments performed 2 experiments Detail