BMRB: 11346

Solution structure of Fn14 CRD domain

Authors

He, F., Dang, W., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Tumor necrosis factor receptor superfamily member 12A

Entity

1. Tumor necrosis factor receptor superfamily member 12A (polymer, Thiol state: all free), 50 monomers, 4953.385 Da Detail

GSSGSSGEQA PGTAPCSRGS SWSADLDKCM DCASCRARPH SDFCLGCAAA

Formula weight

4953.385 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.0 %, Completeness: 86.1 %, Completeness (bb): 84.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.1 % (409 of 475)	85.2 % (208 of 244)	87.4 % (159 of 182)	85.7 % (42 of 49)
Backbone	84.0 % (247 of 294)	83.5 % (86 of 103)	84.0 % (121 of 144)	85.1 % (40 of 47)
Sidechain	89.3 % (201 of 225)	86.5 % (122 of 141)	93.9 % (77 of 82)	100.0 % (2 of 2)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (12 of 12)	100.0 % (1 of 1)
Methyl	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)

1. CRD domain, UNP residues 28-70

GSSGSSGEQA PGTAPCSRGS SWSADLDKCM DCASCRARPH SDFCLGCAAA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.1mM 13C, 15N-labeled {Fn14;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Fn14	[U-13C; U-15N]	protein	1.1 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RPJ, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the cysteine-rich domain in Fn14, a member of the tumor necrosis factor receptor superfamily
He, F., Dang, W., Saito, K., Watanabe, S., Kobayashi, N., Guntert, P., Kigawa, T., Tanaka, A., Muto, Y., Yokoyama, S.
Protein Sci. (2009), 18, 650-656, PubMed 19241374 , DOI 10.1002/pro.49 , Abstract

Related entities 1. Tumor necrosis factor receptor superfamily member 12A, : 1 : 1 : 1 : 5 entities Detail

Interaction partners 1. Tumor necrosis factor receptor superfamily member 12A, : 5 interactors Detail

Experiments performed 2 experiments Detail