BMRB: 11348

Solution structure of the NEUZ domain in KIAA1787 protein

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

KIAA1787 protein

Entity

1. KIAA1787 protein (polymer, Thiol state: all free), 170 monomers, 18161.23 Da Detail

GSSGSSGELH PRTGRLVSLS ACGRTARRQQ PGQEFNHGLV LSREPLRDGR VFTVRIDRKV NSWSGSIEIG VTALDPSVLD FPSSATGLKG GSWVVSGCSV LRDGRSVLEE YGQDLDQLGE GDRVGVERTV AGELRLWVNG RDCGVAATGL PPRVWAVVDL YGKCTQITVL

Formula weight

18161.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 96.3 %, Completeness (bb): 96.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1784 of 1853)	95.6 % (915 of 957)	97.4 % (701 of 720)	95.5 % (168 of 176)
Backbone	96.1 % (967 of 1006)	95.8 % (342 of 357)	96.7 % (470 of 486)	95.1 % (155 of 163)
Sidechain	96.7 % (960 of 993)	95.5 % (573 of 600)	98.4 % (374 of 380)	100.0 % (13 of 13)
Aromatic	91.2 % (93 of 102)	88.2 % (45 of 51)	93.6 % (44 of 47)	100.0 % (4 of 4)
Methyl	98.5 % (199 of 202)	98.0 % (99 of 101)	99.0 % (100 of 101)

1. neuralized domain

GSSGSSGELH PRTGRLVSLS ACGRTARRQQ PGQEFNHGLV LSREPLRDGR VFTVRIDRKV NSWSGSIEIG VTALDPSVLD FPSSATGLKG GSWVVSGCSV LRDGRSVLEE YGQDLDQLGE GDRVGVERTV AGELRLWVNG RDCGVAATGL PPRVWAVVDL YGKCTQITVL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	neuralized domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.6 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2E63, Strand ID: A Detail

---

Release date

2011-09-06

Citation

Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway
He, F., Saito, K., Kobayashi, N., Harada, T., Watanabe, S., Kigawa, T., Guntert, P., Ohara, O., Tanaka, A., Unzai, S., Muto, Y., Yokoyama, S.
J. Mol. Biol. (2009), 393, 478-495, PubMed 19683535 , DOI 10.1016/j.jmb.2009.08.020 , Abstract

Related entities 1. KIAA1787 protein, : 1 : 5 : 154 entities Detail

Interaction partners 1. KIAA1787 protein, : 28 interactors Detail

Experiments performed 2 experiments Detail