BMRB: 11349

Solution structure of LIM domain in LIM-protein 3

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Skeletal muscle LIM-protein 3

Entity

1. LIM domain (polymer, Thiol state: all other bound), 72 monomers, 7917.885 Da Detail

GSSGSSGCQE CKKTIMPGTR KMEYKGSSWH ETCFICHRCQ QPIGTKSFIP KDNQNFCVPC YEKQHASGPS SG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8048.7026 Da

Max. entity weight

7917.885 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS30:ND1	2:ZN1:ZN
4	na	sing	1:CYS33:SG	2:ZN1:ZN
5	na	sing	1:CYS36:SG	2:ZN1:ZN
6	na	sing	1:CYS39:SG	2:ZN1:ZN
7	na	sing	1:CYS57:SG	2:ZN1:ZN
8	na	sing	1:CYS60:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 92.6 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.6 % (755 of 815)	91.7 % (396 of 432)	94.5 % (291 of 308)	90.7 % (68 of 75)
Backbone	90.0 % (380 of 422)	87.8 % (129 of 147)	91.8 % (191 of 208)	89.6 % (60 of 67)
Sidechain	95.2 % (435 of 457)	93.7 % (267 of 285)	97.6 % (160 of 164)	100.0 % (8 of 8)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (34 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)

1. LIM domain

GSSGSSGCQE CKKTIMPGTR KMEYKGSSWH ETCFICHRCQ QPIGTKSFIP KDNQNFCVPC YEKQHASGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.8mM U-15, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 100uM {ZnCl2;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	LIM domain	[U-13C; U-15N]	protein	0.8 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	ZnCl2	natural abundance	salt	100 uM
5	d-DTT	natural abundance	salt	1 mM
6	NaN3	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X4K, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the chicken cysteine-rich protein, CRP1, a double-LIM protein implicated in muscle differentiation
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.
Biochemistry (1999), 38, 5701-5713, PubMed 10231520 , DOI 10.1021/bi982036y , Abstract

Related entities 1. LIM domain, : 1 : 2 : 325 entities Detail

Interaction partners 1. LIM domain, : 111 interactors Detail

Experiments performed 2 experiments Detail