BMRB: 11350

Solution structure of LIM domain in Four and a half LIM domains protein 2

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Skeletal muscle LIM-protein 3

Entity

1. LIM domain (polymer, Thiol state: all other bound), 72 monomers, 7508.278 Da Detail

GSSGSSGCAG CTNPISGLGG TKYISFEERQ WHNDCFNCKK CSLSLVGRGF LTERDDILCP DCGKDISGPS SG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7639.0957 Da

Max. entity weight

7508.278 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS32:ND1	2:ZN1:ZN
4	na	sing	1:CYS35:SG	2:ZN1:ZN
5	na	sing	1:CYS38:SG	2:ZN1:ZN
6	na	sing	1:CYS41:SG	2:ZN1:ZN
7	na	sing	1:CYS59:SG	2:ZN1:ZN
8	na	sing	1:CYS62:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 94.9 %, Completeness (bb): 92.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (719 of 758)	94.9 % (374 of 394)	95.2 % (276 of 290)	93.2 % (69 of 74)
Backbone	92.7 % (395 of 426)	92.2 % (141 of 153)	93.1 % (190 of 204)	92.8 % (64 of 69)
Sidechain	97.2 % (381 of 392)	96.7 % (233 of 241)	97.9 % (143 of 146)	100.0 % (5 of 5)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. LIM domain

GSSGSSGCAG CTNPISGLGG TKYISFEERQ WHNDCFNCKK CSLSLVGRGF LTERDDILCP DCGKDISGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.8mM U-15, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 100uM {ZnCl2;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	LIM domain	[U-13C; U-15N]	protein	0.8 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	ZnCl2	natural abundance	salt	100 uM
5	d-DTT	natural abundance	salt	1 mM
6	NaN3	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.42 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X4L, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of LIM domain in Four and a half LIM domains protein 2
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. LIM domain, : 1 : 266 entities Detail

Interaction partners 1. LIM domain, : 6 interactors Detail

Experiments performed 2 experiments Detail