BMRB: 11351

Solution structure of the NEUZ (NHR) domain in Neuralized from Drosophila melanogaster

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Tarada, T., Yokoyama, S.

Assembly

Protein neuralized

Entity

1. Protein neuralized (polymer, Thiol state: all free), 168 monomers, 18920.08 Da Detail

GSSGSSGPLQ FHSVHGDNIR ISRDGTLARR FESFCRAITF SARPVRINER ICVKFAEISN NWNGGIRFGF TSNDPVTLEG TLPKYACPDL TNRPGFWAKA LHEQYCEKDN ILYYYVNGAG DVIYGINNEE KGVILTGIDT RSLLWTVIDI YGNCTGIEFL DSRIYMYQ

Formula weight

18920.08 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 95.5 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (1860 of 1947)	95.1 % (957 of 1006)	96.2 % (732 of 761)	95.0 % (171 of 180)
Backbone	94.4 % (940 of 996)	94.2 % (327 of 347)	94.5 % (460 of 487)	94.4 % (153 of 162)
Sidechain	96.7 % (1066 of 1102)	95.6 % (630 of 659)	98.4 % (418 of 425)	100.0 % (18 of 18)
Aromatic	98.1 % (206 of 210)	98.1 % (103 of 105)	98.0 % (100 of 102)	100.0 % (3 of 3)
Methyl	100.0 % (176 of 176)	100.0 % (88 of 88)	100.0 % (88 of 88)

1. NEUZ(NHR) domain

GSSGSSGPLQ FHSVHGDNIR ISRDGTLARR FESFCRAITF SARPVRINER ICVKFAEISN NWNGGIRFGF TSNDPVTLEG TLPKYACPDL TNRPGFWAKA LHEQYCEKDN ILYYYVNGAG DVIYGINNEE KGVILTGIDT RSLLWTVIDI YGNCTGIEFL DSRIYMYQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.0mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NEUZ(NHR) domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUE, Strand ID: A Detail

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Release date

2011-09-06

Citation

Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway
He, F., Saito, K., Kobayashi, N., Harada, T., Watanabe, S., Kigawa, T., Guntert, P., Ohara, O., Tanaka, A., Unzai, S., Muto, Y., Yokoyama, S.
J. Mol. Biol. (2009), 393, 478-495, PubMed 19683535 , DOI 10.1016/j.jmb.2009.08.020 , Abstract

Related entities 1. Protein neuralized, : 1 : 3 : 78 entities Detail

Interaction partners 1. Protein neuralized, : 5 interactors Detail

Experiments performed 2 experiments Detail