BMRB: 11357

Solution structure of WW domain in transcription elongation regulator 1

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Transcription elongation regulator 1

Entity

1. Transcription elongation regulator 1 (polymer, Thiol state: all free), 73 monomers, 7853.727 Da Detail

GSSGSSGKAK PVATAPIPGT PWCVVWTGDE RVFFYNPTTR LSMWDRPDDL IGRADVDKII QEPPHKKSGP SSG

Formula weight

7853.727 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 90.0 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (735 of 817)	89.5 % (382 of 427)	91.0 % (292 of 321)	88.4 % (61 of 69)
Backbone	86.7 % (364 of 420)	84.8 % (123 of 145)	87.7 % (185 of 211)	87.5 % (56 of 64)
Sidechain	93.1 % (430 of 462)	91.8 % (259 of 282)	94.9 % (166 of 175)	100.0 % (5 of 5)
Aromatic	97.1 % (66 of 68)	97.1 % (33 of 34)	96.8 % (30 of 31)	100.0 % (3 of 3)
Methyl	96.8 % (60 of 62)	100.0 % (31 of 31)	93.5 % (29 of 31)

1. WW domain

GSSGSSGKAK PVATAPIPGT PWCVVWTGDE RVFFYNPTTR LSMWDRPDDL IGRADVDKII QEPPHKKSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.8mM U-15N, {13C;} 20mM phosphate buffer {NA;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90%H2O,10%D2O

#	Name	Isotope labeling	Type	Concentration
1	WW domain	[U-13C; U-15N]	protein	0.8 mM
2	phosphate buffer NA	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.28 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.28 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DK7, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of WW domain in transcription elongation regulator 1
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. Transcription elongation regulator 1, : 1 : 28 entities Detail

Interaction partners 1. Transcription elongation regulator 1, : 20 interactors Detail

Experiments performed 2 experiments Detail