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BMRB: 11363


11363
1X4S
Solution structure of zinc finger HIT domain in protein FON
Authors
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.
Assembly
Zinc finger HIT domain containing protein 2
Entity
1. Zinc finger HIT domain (polymer, Thiol state: all other bound), 59 monomers, 6084.688 Da Detail

GSSGSSGMEP AGPCGFCPAG EVQPARYTCP RCNAPYCSLR CYRTHGTCAE NFYSGPSSG


2. ZN (non-polymer), 65.409 × 2 Da
Total weight
6215.506 Da
Max. entity weight
6084.688 Da
Entity Connection
na 8 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS14:SG2:ZN1:ZN
2nasing1:CYS17:SG2:ZN1:ZN
3nasing1:CYS37:SG2:ZN1:ZN
4nasing1:CYS41:SG2:ZN1:ZN
5nasing1:CYS29:SG2:ZN1:ZN
6nasing1:CYS32:SG2:ZN1:ZN
7nasing1:HIS45:NE22:ZN1:ZN
8nasing1:CYS48:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
Torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.1 %, Completeness: 89.3 %, Completeness (bb): 86.5 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All89.3 % (535 of 599)89.1 % (278 of 312)90.9 % (211 of 232)83.6 % (46 of 55)
Backbone86.5 % (294 of 340)84.2 % (101 of 120)89.3 % (150 of 168)82.7 % (43 of 52)
Sidechain92.9 % (287 of 309)92.2 % (177 of 192)93.9 % (107 of 114)100.0 % (3 of 3)
Aromatic89.3 % (50 of 56)89.3 % (25 of 28)89.3 % (25 of 28)
Methyl100.0 % (24 of 24)100.0 % (12 of 12)100.0 % (12 of 12)

1. Zinc finger HIT domain

GSSGSSGMEP AGPCGFCPAG EVQPARYTCP RCNAPYCSLR CYRTHGTCAE NFYSGPSSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.8mM U-15, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 100uM {ZnCl2;} 1mM {d-DTT;} 0.02% NaN3


#NameIsotope labelingTypeConcentration
1Zinc finger HIT domain[U-13C; U-15N]protein0.8 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4ZnCl2natural abundancesalt100 uM
5d-DTTnatural abundancesalt1 mM
6NaN3natural abundancesalt0.02 %
7H2Osolvent90 %
8D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.16 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1X4S, Strand ID: A Detail

Release date
2011-09-06
Citation
Solution structure of the zinc finger HIT domain in protein FON
He, F., Umehara, T., Tsuda, K., Inoue, M., Kigawa, T., Matsuda, T., Yabuki, T., Aoki, M., Seki, E., Terada, T., Shirouzu, M., Tanaka, A., Sugano, S., Muto, Y., Yokoyama, S.
Protein Sci. (2007), 16, 1577-1587, PubMed 17656577 , DOI 10.1110/ps.062635107 ,
Related entities 1. Zinc finger HIT domain, : 1 : 5 entities Detail
More details for 6 related entities
Experiments performed 2 experiments Detail
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