BMRB: 11364

Solution structure of the first SURP domain of human splicing factor SF3a120

Authors

Kuwasako, K., He, F., Inoue, M., Tanaka, A., Sugano, S., Guntert, P., Muto, Y., Yokoyama, S.

Assembly

Splicing factor 3 subunit 1

Entity

1. Splicing factor 3 subunit 1 (polymer, Thiol state: not present), 64 monomers, 7466.129 Da Detail

GEVRNIVDKT ASFVARNGPE FEARIRQNEI NNPKFNFLNP NDPYHAYYRH KVSEFKEGKA QEPS

Formula weight

7466.129 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS, RESTRAINTED ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (763 of 777)	98.1 % (405 of 413)	99.0 % (292 of 295)	95.7 % (66 of 69)
Backbone	98.1 % (367 of 374)	97.6 % (123 of 126)	99.5 % (188 of 189)	94.9 % (56 of 59)
Sidechain	98.5 % (457 of 464)	98.3 % (282 of 287)	98.8 % (165 of 167)	100.0 % (10 of 10)
Aromatic	95.1 % (78 of 82)	95.1 % (39 of 41)	95.1 % (39 of 41)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. SURP domain

GEVRNIVDKT ASFVARNGPE FEARIRQNEI NNPKFNFLNP NDPYHAYYRH KVSEFKEGKA QEPS

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 278 (±0.1) K, pH 7.0 (±0.05), Details 2.0mM U-15N, 13C {PROTEIN;} 20mM soldium {phosphate;} 1mM {d-DTT;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SURP domain	[U-13C; U-15N]	protein	2.0 mM
2	soldium phosphate	natural abundance	buffer	20 mM
3	d-DTT	natural abundance	salt	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.6 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DT6, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP
Kuwasako, K., He, F., Inoue, M., Tanaka, A., Sugano, S., Guntert, P., Muto, Y., Yokoyama, S.
Structure (2006), 14, 1677-1689, PubMed 17098193 , DOI 10.1016/j.str.2006.09.009 , Abstract

Related entities 1. Splicing factor 3 subunit 1, : 1 : 6 : 131 entities Detail

Interaction partners 1. Splicing factor 3 subunit 1, : 62 interactors Detail

Experiments performed 2 experiments Detail