BMRB: 11368

Solution structure of the C2H2 type zinc finger (region 656-688) of human Zinc finger protein 95 homolog

Authors

Takahashi, M., Kuwasako, K., Tsuda, K., Tanabe, W., Harada, T., Watanabe, S., Tochio, N., Muto, Y., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 95 homolog

Entity

1. ZF-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4954.368 Da Detail

GSSGSSGSRE KSHQCRECGE IFFQYVSLIE HQVLHMGQKN SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

5019.7773 Da

Max. entity weight

4954.368 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 86.1 %, Completeness (bb): 84.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.1 % (434 of 504)	83.8 % (222 of 265)	91.5 % (173 of 189)	78.0 % (39 of 50)
Backbone	84.3 % (231 of 274)	79.6 % (78 of 98)	88.5 % (116 of 131)	82.2 % (37 of 45)
Sidechain	88.5 % (238 of 269)	86.2 % (144 of 167)	94.8 % (92 of 97)	40.0 % (2 of 5)
Aromatic	95.0 % (38 of 40)	95.0 % (19 of 20)	95.0 % (19 of 20)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. ZF-C2H2

GSSGSSGSRE KSHQCRECGE IFFQYVSLIE HQVLHMGQKN SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.0mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ZF-C2H2	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YSO, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the C2H2 type zinc finger (region 656-688) of human Zinc finger protein 95 homolog
Takahashi, M., Kuwasako, K., Tsuda, K., Tanabe, W., Harada, T., Watanabe, S., Tochio, N., Muto, Y., Kigawa, T., Yokoyama, S.

Related entities 1. ZF-C2H2, : 1 : 112 entities Detail

Experiments performed 2 experiments Detail