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BMRB: 11369


11369
2YSP
Solution structure of the C2H2 type zinc finger (region 507-539) of human Zinc finger protein 224
Authors
Takahashi, M., Kuwasako, K., Tsuda, K., Tanabe, W., Harada, T., Watanabe, S., Tochio, N., Muto, Y., Kigawa, T., Yokoyama, S.
Assembly
Zinc finger protein 224
Entity
1. ZF-C2H2 (polymer, Thiol state: all other bound), 46 monomers, 4803.202 Da Detail

GSSGSSGTGE KPYKCEKCGK GYNSKFNLDM HQKVHTGERP SGPSSG


2. ZN (non-polymer), 65.409 Da
Total weight
4868.6113 Da
Max. entity weight
4803.202 Da
Entity Connection
na 4 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS15:SG2:ZN1:ZN
2nasing1:CYS18:SG2:ZN1:ZN
3nasing1:HIS31:NE22:ZN1:ZN
4nasing1:HIS35:NE22:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 87.0 %, Completeness: 88.9 %, Completeness (bb): 84.1 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All88.9 % (439 of 494)89.0 % (234 of 263)89.7 % (166 of 185)84.8 % (39 of 46)
Backbone84.1 % (227 of 270)82.7 % (81 of 98)85.3 % (110 of 129)83.7 % (36 of 43)
Sidechain93.9 % (245 of 261)92.7 % (153 of 165)95.7 % (89 of 93)100.0 % (3 of 3)
Aromatic100.0 % (34 of 34)100.0 % (17 of 17)100.0 % (17 of 17)
Methyl100.0 % (12 of 12)100.0 % (6 of 6)100.0 % (6 of 6)

1. ZF-C2H2

GSSGSSGTGE KPYKCEKCGK GYNSKFNLDM HQKVHTGERP SGPSSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details about 1.0mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O


#NameIsotope labelingTypeConcentration
1ZF-C2H2[U-13C; U-15N]protein1.0 (±0.1) mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.03 ppm, Outliers: 5 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2YSP, Strand ID: A Detail

Release date
2011-09-06
Citation
Solution structure of the C2H2 type zinc finger (region 507-539)of human Zinc finger protein 224
Takahashi, M., Kuwasako, K., Tsuda, K., Tanabe, W., Harada, T., Watanabe, S., Tochio, N., Muto, Y., Kigawa, T., Yokoyama, S.
Related entities 1. ZF-C2H2, : 1 : 174 entities Detail
More details for 175 related entities
Experiments performed 2 experiments Detail
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