BMRB: 11377

Solution structure of the first C2H2 type zinc finger domain of Zinc finger protein 278

Authors

Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

POZ-, AT hook-, and zinc finger-containing protein 1

Entity

1. zinc finger domain (polymer, Thiol state: all other bound), 66 monomers, 6727.474 Da Detail

GSSGSSGLRE AGILPCGLCG KVFTDANRLR QHEAQHGVTS LQLGYIDLPP PRLGENGLPI SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

6792.8833 Da

Max. entity weight

6727.474 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS16:SG	2:ZN1:ZN
2	na	sing	1:CYS19:SG	2:ZN1:ZN
3	na	sing	1:HIS32:NE2	2:ZN1:ZN
4	na	sing	1:HIS36:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dyanamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.4 %, Completeness: 88.3 %, Completeness (bb): 85.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.3 % (619 of 701)	88.5 % (324 of 366)	88.5 % (239 of 270)	86.2 % (56 of 65)
Backbone	85.4 % (328 of 384)	86.2 % (119 of 138)	84.4 % (157 of 186)	86.7 % (52 of 60)
Sidechain	91.6 % (340 of 371)	89.9 % (205 of 228)	94.9 % (131 of 138)	80.0 % (4 of 5)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	95.5 % (63 of 66)	93.9 % (31 of 33)	97.0 % (32 of 33)

1. zinc finger domain

GSSGSSGLRE AGILPCGLCG KVFTDANRLR QHEAQHGVTS LQLGYIDLPP PRLGENGLPI SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.89mM 13C-15N PROTEIN, 20mM d-Tris-HCl(pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O, 0.05mM ZnCl2, 1mM IDA

#	Name	Isotope labeling	Type	Concentration
1	zinc finger domain	[U-13C; U-15N]	protein	0.89 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EPP, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the first C2H2 type zinc finger domain of Zinc finger protein 278
Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Related entities 1. zinc finger domain, : 1 : 4 : 9 entities Detail

Interaction partners 1. zinc finger domain, : 16 interactors Detail

Experiments performed 2 experiments Detail