BMRB: 11378

Solution structure of the third zinc finger domain of Zinc finger protein 278

Authors

Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

POZ-, AT hook-, and zinc finger-containing protein 1

Entity

1. zinc finger domain (polymer, Thiol state: all other bound), 45 monomers, 4716.177 Da Detail

GSSGSSGEKP YSCPVCGLRF KRKDRMSYHV RSHDGSVGKS GPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4781.586 Da

Max. entity weight

4716.177 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dyanamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.4 %, Completeness: 85.7 %, Completeness (bb): 80.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.7 % (409 of 477)	84.2 % (213 of 253)	89.0 % (162 of 182)	81.0 % (34 of 42)
Backbone	80.3 % (212 of 264)	74.7 % (71 of 95)	84.3 % (107 of 127)	81.0 % (34 of 42)
Sidechain	92.0 % (230 of 250)	89.9 % (142 of 158)	95.7 % (88 of 92)
Aromatic	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (8 of 8)

1. zinc finger domain

GSSGSSGEKP YSCPVCGLRF KRKDRMSYHV RSHDGSVGKS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.97mM 13C-15N {PROTEIN;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2, 1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zinc finger domain	[U-13C; U-15N]	protein	0.97 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.2 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EPQ, Strand ID: A Detail

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Release date

2011-09-06

Citation

Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4
Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.
J. Biol. Chem. (2003), 278, 20225-20234, PubMed 12644454 , DOI 10.1074/jbc.M300459200 , Abstract

Related entities 1. zinc finger domain, : 1 : 211 entities Detail

Experiments performed 2 experiments Detail