BMRB: 11379

Solution structure of the secound zinc finger domain of Zinc finger protein 278

Authors

Suzuki, S., Tanabe, W., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

POZ-, AT hook-, and zinc finger-containing protein 1

Entity

1. zinc finger domain, UNP residues 350-384 (polymer, Thiol state: all other bound), 48 monomers, 5149.657 Da Detail

GSSGSSGRTR KQVACEICGK IFRDVYHLNR HKLSHSGEKP YSSGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

5215.0664 Da

Max. entity weight

5149.657 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 72.9 %, Completeness: 70.5 %, Completeness (bb): 64.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	70.5 % (370 of 525)	67.1 % (186 of 277)	77.5 % (155 of 200)	60.4 % (29 of 48)
Backbone	64.8 % (184 of 284)	59.4 % (60 of 101)	70.1 % (96 of 137)	60.9 % (28 of 46)
Sidechain	77.3 % (218 of 282)	71.6 % (126 of 176)	87.5 % (91 of 104)	50.0 % (1 of 2)
Aromatic	89.5 % (34 of 38)	89.5 % (17 of 19)	89.5 % (17 of 19)
Methyl	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)

1. zinc finger domain, UNP residues 350-384

GSSGSSGRTR KQVACEICGK IFRDVYHLNR HKLSHSGEKP YSSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.25mM 13C-15N {PROTEIN;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2+1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zinc finger domain, UNP residues 350-384	[U-13C; U-15N]	protein	0.25 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EPR, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the secound zinc finger domain of Zinc finger protein 278
Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Related entities 1. zinc finger domain, UNP residues 350-384, : 1 : 92 entities Detail

Experiments performed 2 experiments Detail