BMRB: 11380

Solution structure of the 4th zinc finger domain of Zinc finger protein 278

Authors

Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

POZ-, AT hook-, and zinc finger-containing protein 1

Entity

1. zinc finger domain, UNP residues 408-448 (polymer, Thiol state: free and other bound), 54 monomers, 5637.184 Da Detail

GSSGSSGSVG KPYICQSCGK GFSRPDHLNG HIKQVHTSER PHKCQVWVSG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

5702.5933 Da

Max. entity weight

5637.184 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS36:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.5 %, Completeness: 84.5 %, Completeness (bb): 79.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.5 % (491 of 581)	83.9 % (256 of 305)	86.4 % (191 of 221)	80.0 % (44 of 55)
Backbone	79.1 % (250 of 316)	75.2 % (85 of 113)	82.4 % (126 of 153)	78.0 % (39 of 50)
Sidechain	90.3 % (280 of 310)	89.1 % (171 of 192)	92.0 % (104 of 113)	100.0 % (5 of 5)
Aromatic	91.3 % (42 of 46)	91.3 % (21 of 23)	90.9 % (20 of 22)	100.0 % (1 of 1)
Methyl	93.3 % (28 of 30)	93.3 % (14 of 15)	93.3 % (14 of 15)

1. zinc finger domain, UNP residues 408-448

GSSGSSGSVG KPYICQSCGK GFSRPDHLNG HIKQVHTSER PHKCQVWVSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.08mM 13C-15N {PROTEIN;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2+1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zinc finger domain, UNP residues 408-448	[U-13C; U-15N]	protein	1.08 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EPS, Strand ID: A Detail

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Release date

2011-09-06

Citation

Solution structure of the 4th zinc finger domain of Zinc finger protein 278
Tanabe, W., Suzuki, S., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Related entities 1. zinc finger domain, UNP residues 408-448, : 1 : 106 entities Detail

Experiments performed 2 experiments Detail