BMRB: 11417

Solution structure of the J domain of DnaJ homolog subfamily B member 8

Authors

Ohnishi, S., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

DnaJ homolog subfamily B member 8

Entity

1. DnaJ homolog subfamily B member 8 (polymer, Thiol state: all free), 92 monomers, 9929.828 Da Detail

GSSGSSGMAN YYEVLGVQAS ASPEDIKKAY RKLALRWHPD KNPDNKEEAE KKFKLVSEAY EVLSDSKKRS LYDRAGCDSW RAGGGASGPS SG

Formula weight

9929.828 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 94.5 %, Completeness (bb): 94.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (964 of 1020)	92.7 % (497 of 536)	97.2 % (379 of 390)	93.6 % (88 of 94)
Backbone	94.7 % (515 of 544)	93.7 % (178 of 190)	95.9 % (255 of 266)	93.2 % (82 of 88)
Sidechain	94.8 % (529 of 558)	92.2 % (319 of 346)	99.0 % (204 of 206)	100.0 % (6 of 6)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. DnaJ domain

GSSGSSGMAN YYEVLGVQAS ASPEDIKKAY RKLALRWHPD KNPDNKEEAE KKFKLVSEAY EVLSDSKKRS LYDRAGCDSW RAGGGASGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.3mM protein U-15N, {13C;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DnaJ domain	[U-13C; U-15N]	protein	1.3 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMX, Strand ID: A Detail

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Release date

2010-10-07

Citation

Solution structure of the J domain of DnaJ homolog subfamily B member 8
Ohnishi, S., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. DnaJ homolog subfamily B member 8, : 1 : 2 : 82 entities Detail

Interaction partners 1. DnaJ homolog subfamily B member 8, : 1 interactors Detail

Experiments performed 2 experiments Detail