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BMRB: 11419

2RRH

NMR structure of vasoactive intestinal peptide in Methanol

Authors

Umetsu, Y., Tenno, T., Goda, N., Ikegami, T., Hiroaki, H.

Assembly

vasoactive intestinal peptide

Entity

1. vasoactive intestinal peptide (polymer, Thiol state: not present), 29 monomers, 3383.833 Da Detail

HSDAVFTDNY TRLRKQMAVK KYLNSILNG

Formula weight

3383.833 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 90.3 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.3 % (317 of 351)	89.6 % (164 of 183)	91.9 % (124 of 135)	87.9 % (29 of 33)
Backbone	91.4 % (159 of 174)	89.8 % (53 of 59)	91.9 % (79 of 86)	93.1 % (27 of 29)
Sidechain	90.2 % (185 of 205)	89.5 % (111 of 124)	93.5 % (72 of 77)	50.0 % (2 of 4)
Aromatic	80.0 % (24 of 30)	80.0 % (12 of 15)	80.0 % (12 of 15)
Methyl	93.8 % (30 of 32)	87.5 % (14 of 16)	100.0 % (16 of 16)

1. VIP

HSDAVFTDNY TRLRKQMAVK KYLNSILNG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2RRH, Strand ID: A Detail

Release date

2011-05-18

Citation

Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments
Umetsu, Y., Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., Hiroaki, H.
Biochim. Biophys. Acta (2011), 1814, 724-730, PubMed 21439408 , DOI 10.1016/j.bbapap.2011.03.009 , Abstract

Related entities 1. vasoactive intestinal peptide, : 1 : 9 : 3 : 74 entities Detail

Interaction partners 1. vasoactive intestinal peptide, : 7 interactors Detail

More details for 7 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

4 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

6 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

7 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	VIP	[U-13C; U-15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	20 mM
3	methanol	[U-2H]		50 %

nullKeywords peptide hormone