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BMRB: 11420

2RRI

NMR structure of vasoactive intestinal peptide in DPC Micelle

Authors

Goda, N., Umetsu, Y., Tenno, T., Shirakawa, M., Ikegami, T., Hiroaki, H.

Assembly

Vasoactive intestinal peptide

Entity

1. Vasoactive intestinal peptide (polymer, Thiol state: not present), 29 monomers, 3383.833 Da Detail

HSDAVFTDNY TRLRKQMAVK KYLNSILNG

Formula weight

3383.833 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 94.0 %, Completeness (bb): 94.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.0 % (330 of 351)	94.0 % (172 of 183)	94.1 % (127 of 135)	93.9 % (31 of 33)
Backbone	94.8 % (165 of 174)	94.9 % (56 of 59)	95.3 % (82 of 86)	93.1 % (27 of 29)
Sidechain	93.7 % (192 of 205)	93.5 % (116 of 124)	93.5 % (72 of 77)	100.0 % (4 of 4)
Aromatic	80.0 % (24 of 30)	80.0 % (12 of 15)	80.0 % (12 of 15)
Methyl	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)

1. Vasoactive intestinal peptide

HSDAVFTDNY TRLRKQMAVK KYLNSILNG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2RRI, Strand ID: A Detail

Release date

2011-05-18

Citation

Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments
Umetsu, Y., Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., Hiroaki, H.
Biochim. Biophys. Acta (2011), 1814, 724-730, PubMed 21439408 , DOI 10.1016/j.bbapap.2011.03.009 , Abstract

Related entities 1. Vasoactive intestinal peptide, : 1 : 9 : 3 : 74 entities Detail

Interaction partners 1. Vasoactive intestinal peptide, : 7 interactors Detail

More details for 7 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

4 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

6 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

7 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	vasoactive intestinal peptide	[U-13C; U-15N]	protein	0.5 mM
2	DPC	natural abundance	lipid	1 %
3	potassium phosphate	natural abundance	buffer	50 mM

nullKeywords HORMONE