BMRBj - BMREntryMaster

Found 1 Document (3.677 seconds)

BMRB: 11424

2RRM

Interplay between phosphatidyl-inositol-phosphates and claudins upon binding to the 1st PDZ domain of zonula ocludens 1

Authors

Hiroaki, H., Satomura, K., Goda, N., Umetsu, Y., Taniguchi, R., Ikegami, T., Furuse, M.

Assembly

tight junction protein ZO-1

Entity

1. tight junction protein ZO-1 (polymer, Thiol state: not present), 100 monomers, 10800.02 Da Detail

GPLGSDHIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG ETSIVISDVL KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK NAKITIRRKK

Formula weight

10800.02 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 84.9 %, Completeness (bb): 92.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (957 of 1127)	84.5 % (497 of 588)	86.3 % (372 of 431)	81.5 % (88 of 108)
Backbone	92.6 % (548 of 592)	91.9 % (192 of 209)	93.7 % (269 of 287)	90.6 % (87 of 96)
Sidechain	78.9 % (491 of 622)	80.5 % (305 of 379)	80.1 % (185 of 231)	8.3 % (1 of 12)
Aromatic	29.2 % (21 of 72)	47.2 % (17 of 36)	11.4 % (4 of 35)	0.0 % (0 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. the first PDZ domain of mouse ZO-1

GPLGSDHIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG ETSIVISDVL KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK NAKITIRRKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	ZO-1(PDZ1)	[U-13C; U-15N]	protein	0.7 mM
2	MES	natural abundance	buffer	20 mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RRM, Strand ID: A Detail

Release date

2011-06-30

Citation

1H, 13C, and 15N resonance assignment of the first PDZ domain of mouse ZO-1
Umetsu, Y., Goda, N., Taniguchi, R., Satomura, K., Ikegami, T., Furuse, M., Hiroaki, H.
Biomol. NMR Assign. (2011), 5, 207-210, PubMed 21431884 , DOI 10.1007/s12104-011-9301-x , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P39447 released on 1995-02-01
Title ZO1_MOUSE Entity Tight junction protein ZO-1

Related entities 1. tight junction protein ZO-1, : 1 : 12 : 393 entities Detail

Interaction partners 1. tight junction protein ZO-1, : 63 interactors Detail

More details for 63 interactors identified by 30 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC