BMRB: 11438

DNA oligomer containing ethylene cross-linked cyclic 2'-deoxyuridylate dimer

Authors

Furuita, K., Murata, S., Jee, J., Ichikawa, S., Matsuda, A., Kojima, C.

Assembly

Protein

Entity

1. Protein (polymer, Thiol state: not present), 28 monomers, 8667.535 Da Detail

CCTTCATTAC ATCCGGATGT AATGAAGG

Formula weight

8667.535 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 68.4 %, Completeness (bb): 57.1 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	68.4 % (186 of 272)	68.4 % (186 of 272)
Suger, PO4	57.1 % (112 of 196)	57.1 % (112 of 196)
Nucleobase	97.4 % (74 of 76)	97.4 % (74 of 76)
Aromatic	96.4 % (54 of 56)	96.4 % (54 of 56)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. Ethylene-DNA

CCTTCATTAC ATCCGGATGT AATGAAGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Ethylene-DNA	natural abundance	DNA	0.7 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

Sample #2

Solvent system 100% D2O, Temperature 283 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
6	Ethylene-DNA	natural abundance	DNA	0.7 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	sodium chloride	natural abundance	salt	100 mM
9	D2O	natural abundance	solvent	100 %

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Release date

2011-06-21

Citation

Structural feature of bent DNA recognized by HMGB1
Furuita, K., Murata, S., Jee, J., Ichikawa, S., Matsuda, A., Kojima, C.
J. Am. Chem. Soc. (2011), 133, 5788-5790, PubMed 21443191 , DOI 10.1021/ja2013399 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 5 experiments Detail