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BMRB: 11450

2RS2

1H, 13C, and 15N Chemical Shift Assignments for Musashi1 RBD1:r(GUAGU) complex

Authors

Ohyama, T., Nagata, T., Tsuda, K., Imai, T., Okano, H., Yamazaki, T., Katahira, M.

Assembly

RNA-binding protein Musashi homolog 1

Entity

1. RNA-binding protein Musashi homolog 1, entity 1 (polymer, Thiol state: all free), 109 monomers, 12180.72 Da Detail

MGSSHHHHHH SSGLVPRGSH MGSSGCKMFI GGLSWQTTQE GLREYFGQFG EVKECLVMRD PLTKRSRGFG FVTFMDQAGV DKVLAQSRHE LDSKTIDPKV AFPRRAQPK

2. RNA-binding protein Musashi homolog 1, entity 2 (polymer, Thiol state: not present), 5 monomers, 1649.964 Da Detail

GUAGU

Total weight

13830.684 Da

Max. entity weight

12180.72 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.2 %, Completeness: 75.5 %, Completeness (bb): 72.2 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	75.5 % (1010 of 1338)	74.9 % (526 of 702)	75.4 % (393 of 521)	79.1 % (91 of 115)
Backbone	72.2 % (505 of 699)	67.2 % (172 of 256)	73.5 % (249 of 339)	80.8 % (84 of 104)
Sidechain	79.3 % (583 of 735)	79.4 % (354 of 446)	79.9 % (222 of 278)	63.6 % (7 of 11)
Aromatic	61.0 % (89 of 146)	61.6 % (45 of 73)	63.2 % (43 of 68)	20.0 % (1 of 5)
Methyl	90.2 % (74 of 82)	90.2 % (37 of 41)	90.2 % (37 of 41)

1. entity 1

MGSSHHHHHH SSGLVPRGSH MGSSGCKMFI GGLSWQTTQE GLREYFGQFG EVKECLVMRD PLTKRSRGFG FVTFMDQAGV DKVLAQSRHE LDSKTIDPKV AFPRRAQPK

2. entity 2

GUAGU

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Msi1 RBD1	[U-13C; U-15N]	protein	300 uM
2	RNA	natural abundance	RNA	300 uM
3	MES	natural abundance		20 mM
4	DTT	natural abundance		5 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RS2, Strand ID: A Detail

Release date

2012-08-16

Citation

Structure of Musashi1 in a complex with target RNA: the role of aromatic stacking interactions
Ohyama, T., Nagata, T., Tsuda, K., Imai, T., Okano, H., Yamazaki, T., Katahira, M.
Nucleic Acids Res. (2012), 40, 3218-3231, PubMed 22140116 , DOI 10.1093/nar/gkr1139 , Abstract

Related entities 1. RNA-binding protein Musashi homolog 1, entity 1, : 1 : 87 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC