BMRB: 11456

Solution structure of the N-terminal dsRBD from RNA helicase A

Authors

Nagata, T., Muto, Y., Tsuda, K., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

Solution structure of the N-terminal dsRBD from RNA helicase A

Entity

1. Solution structure of the N-terminal dsRBD from RNA helicase A (polymer, Thiol state: all free), 99 monomers, 10719.00 Da Detail

GSSGSSGIKN FLYAWCGKRK MTPAYEIRAV GNKNRQKFMC EVRVEGFNYA GMGNSTNKKD AQSNAARDFV NYLVRINEVK SEEVPAVGIV PPPSGPSSG

Formula weight

10719.0 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 89.8 %, Completeness (bb): 88.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (1007 of 1121)	89.3 % (526 of 589)	89.5 % (382 of 427)	94.3 % (99 of 105)
Backbone	88.8 % (517 of 582)	88.7 % (180 of 203)	87.4 % (250 of 286)	93.5 % (87 of 93)
Sidechain	90.6 % (568 of 627)	89.6 % (346 of 386)	91.7 % (210 of 229)	100.0 % (12 of 12)
Aromatic	94.0 % (79 of 84)	88.1 % (37 of 42)	100.0 % (41 of 41)	100.0 % (1 of 1)
Methyl	98.8 % (79 of 80)	100.0 % (40 of 40)	97.5 % (39 of 40)

1. entity

GSSGSSGIKN FLYAWCGKRK MTPAYEIRAV GNKNRQKFMC EVRVEGFNYA GMGNSTNKKD AQSNAARDFV NYLVRINEVK SEEVPAVGIV PPPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]	protein	1.3 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	natural abundance		1 mM
5	sodium azide	natural abundance		0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RS6, Strand ID: A Detail

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Release date

2012-08-28

Citation

Solution structures of the double-stranded RNA-binding domains from RNA helicase A
Nagata, T., Tsuda, K., Kobayashi, N., Shirouzu, M., Kigawa, T., Guntert, P., Yokoyama, S., Muto, Y.
Proteins (2012), 80, 1699-1706, PubMed 22454253 , DOI 10.1002/prot.24059 , Abstract

Related entities 1. Solution structure of the N-terminal dsRBD from RNA helicase A, : 1 : 1 : 3 : 22 entities Detail

Interaction partners 1. Solution structure of the N-terminal dsRBD from RNA helicase A, : 19 interactors Detail

Experiments performed 2 experiments Detail