BMRB: 11458

Solution structure of the N-terminal RNA recognition motif of NonO

Authors

Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

N-terminal RNA recognition motif of NonO

Entity

1. N-terminal RNA recognition motif of NonO (polymer, Thiol state: all free), 99 monomers, 10891.27 Da Detail

GSSGSSGGEK TFTQRSRLFV GNLPPDITEE EMRKLFEKYG KAGEVFIHKD KGFGFIRLET RTLAEIAKVE LDNMPLRGKQ LRVRFACHSA SLTSGPSSG

Formula weight

10891.27 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 88.4 %, Completeness (bb): 84.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.4 % (1011 of 1144)	89.2 % (536 of 601)	89.0 % (395 of 444)	80.8 % (80 of 99)
Backbone	84.3 % (494 of 586)	84.0 % (173 of 206)	86.0 % (245 of 285)	80.0 % (76 of 95)
Sidechain	92.2 % (595 of 645)	91.9 % (363 of 395)	92.7 % (228 of 246)	100.0 % (4 of 4)
Aromatic	86.0 % (74 of 86)	86.0 % (37 of 43)	86.0 % (37 of 43)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. entity

GSSGSSGGEK TFTQRSRLFV GNLPPDITEE EMRKLFEKYG KAGEVFIHKD KGFGFIRLET RTLAEIAKVE LDNMPLRGKQ LRVRFACHSA SLTSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]	protein	1.2 mM
2	TRIS	[U-100% 2H]		20 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.784 ppm	internal	indirect	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RS8, Strand ID: A Detail

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Release date

2012-12-17

Citation

Solution structure of the N-terminal RNA recognition motif of NonO
Nagata, T.
Not known

Related entities 1. N-terminal RNA recognition motif of NonO, : 1 : 1 : 11 : 145 entities Detail

Interaction partners 1. N-terminal RNA recognition motif of NonO, : 2 interactors Detail

Experiments performed 2 experiments Detail