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Found 1 Document (1.307 seconds)

BMRB: 11463

2RS9

Solution structure of the bromodomain of human BRPF1 in complex with histone H4K5ac peptide

Authors

Qin, X., Nagashima, T., Umehara, T., Hayashi, F., Yokoyama, S.

Assembly

Bromodomain of human BRPF1 in complex with histone H4K5ac peptide

Entity

1. An acetylated-lysine 5 of histone 4 peptide (polymer, Thiol state: all free), 10 monomers, 958.0756 Da Detail

SGRGXGGKGL

2. Bromodomain (polymer, Thiol state: all free), 121 monomers, 13760.38 Da Detail

GSSGSSGFLI LLRKTLEQLQ EKDTGNIFSE PVPLSEVPDY LDHIKKPMDF FTMKQNLEAY RYLNFDDFEE DFNLIVSNCL KYNAKDTIFY RAAVRLREQG GAVLRQARRQ AEKMGSGPSS G

Total weight

14718.455 Da

Max. entity weight

13760.38 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 96.5 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1468 of 1522)	98.6 % (789 of 800)	94.7 % (554 of 585)	91.2 % (125 of 137)
Backbone	94.2 % (725 of 770)	96.3 % (259 of 269)	93.9 % (353 of 376)	90.4 % (113 of 125)
Sidechain	98.5 % (855 of 868)	99.8 % (530 of 531)	96.3 % (313 of 325)	100.0 % (12 of 12)
Aromatic	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)
Methyl	98.3 % (116 of 118)	100.0 % (59 of 59)	96.6 % (57 of 59)

1. An acetylated-lysine 5 of histone 4 peptide

SGRGXGGKGL

2. Bromodomain

GSSGSSGFLI LLRKTLEQLQ EKDTGNIFSE PVPLSEVPDY LDHIKKPMDF FTMKQNLEAY RYLNFDDFEE DFNLIVSNCL KYNAKDTIFY RAAVRLREQG GAVLRQARRQ AEKMGSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5, Details 50mM Phosphorate Buffer(pH 6.5); 50mMNaCl; 5mM d-DTT; 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	1.5 mM
2	entity_2	[U-99% 13C; U-99% 15N]	protein	0.54 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	sodium phosphate	natural abundance	buffer	50 mM
6	NaCl	natural abundance	salt	50 mM
7	d-DTT	natural abundance	salt	5 mM
8	NaN3	natural abundance	salt	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.89 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.89 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.89 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.89 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RS9, Strand ID: A, B Detail

Release date

2013-03-25

Citation

Site-specific histone recognition by the bromodomain of Brpf1 and the role in MOZ/MORF histone acetyltransferase complexes
Qin, X., Hayashi, F., Yokoyama, S.
Not known

Related entities 2. Bromodomain, : 4 : 53 entities Detail

Interaction partners 2. Bromodomain, : 18 interactors Detail

More details for 18 interactors identified by 3 citations

Experiments performed 4 experiments Detail

1 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5, Details 50mM Phosphorate Buffer(pH 6.5); 50mMNaCl; 5mM d-DTT; 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	1.5 mM
2	entity_2	[U-99% 13C; U-99% 15N]	protein	0.54 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	sodium phosphate	natural abundance	buffer	50 mM
6	NaCl	natural abundance	salt	50 mM
7	d-DTT	natural abundance	salt	5 mM
8	NaN3	natural abundance	salt	0.02 %

2 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5, Details 50mM Phosphorate Buffer(pH 6.5); 50mMNaCl; 5mM d-DTT; 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	1.5 mM
2	entity_2	[U-99% 13C; U-99% 15N]	protein	0.54 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	sodium phosphate	natural abundance	buffer	50 mM
6	NaCl	natural abundance	salt	50 mM
7	d-DTT	natural abundance	salt	5 mM
8	NaN3	natural abundance	salt	0.02 %

3 13C-edited (F1)

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5, Details 50mM Phosphorate Buffer(pH 6.5); 50mMNaCl; 5mM d-DTT; 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	1.5 mM
2	entity_2	[U-99% 13C; U-99% 15N]	protein	0.54 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	sodium phosphate	natural abundance	buffer	50 mM
6	NaCl	natural abundance	salt	50 mM
7	d-DTT	natural abundance	salt	5 mM
8	NaN3	natural abundance	salt	0.02 %

4 13C/15N-filtered (F3) 3D NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5, Details 50mM Phosphorate Buffer(pH 6.5); 50mMNaCl; 5mM d-DTT; 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	1.5 mM
2	entity_2	[U-99% 13C; U-99% 15N]	protein	0.54 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	sodium phosphate	natural abundance	buffer	50 mM
6	NaCl	natural abundance	salt	50 mM
7	d-DTT	natural abundance	salt	5 mM
8	NaN3	natural abundance	salt	0.02 %

nullKeywords BR140 Protein, Bromodomain and PHD finger-containing protein 1, complexe, Histone, Human BRPF1 protein, transcription activator

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Found 1 Document (1.307 seconds)

BMRB: 11463

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 2. Bromodomain, : 4 : 53 entities Detail

Interaction partners 2. Bromodomain, : 18 interactors Detail

Experiments performed 4 experiments Detail

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