BMRB: 11468

Solution STRUCTURE OF THE BOMBYX MORI LYSOZYME

Authors

Sato, M., Tochio, N., Aizawa, T.

Assembly

THE BOMBYX MORI LYSOZYME

Entity

1. THE BOMBYX MORI LYSOZYME (polymer), 120 monomers, 13808.43 Da Detail

GKTFTRCGLV HELRKHGFEE NLMRNWVCLV EHESSRDTSK TNTNRNGSKD YGLFQINDRY WCSKGASPGK DCNVKCSDLL TDDITKAAKC AKKIYKRHRF DAWYGWKNHC QGSLPDISSC

Formula weight

13808.43 Da

Source organism

Bombyx mori

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.5 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.5 % (1384 of 1405)	97.6 % (719 of 737)	99.4 % (533 of 536)	100.0 % (132 of 132)
Backbone	99.0 % (709 of 716)	98.4 % (243 of 247)	99.1 % (348 of 351)	100.0 % (118 of 118)
Sidechain	98.1 % (785 of 800)	97.1 % (476 of 490)	99.7 % (295 of 296)	100.0 % (14 of 14)
Aromatic	97.9 % (137 of 140)	95.7 % (67 of 70)	100.0 % (66 of 66)	100.0 % (4 of 4)
Methyl	95.5 % (84 of 88)	93.2 % (41 of 44)	97.7 % (43 of 44)

1. THE BOMBYX MORI LYSOZYME

GKTFTRCGLV HELRKHGFEE NLMRNWVCLV EHESSRDTSK TNTNRNGSKD YGLFQINDRY WCSKGASPGK DCNVKCSDLL TDDITKAAKC AKKIYKRHRF DAWYGWKNHC QGSLPDISSC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 3.8

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	natural abundance		20 mM
2	sodium chloride	natural abundance		100 mM
3	sodium azide	natural abundance		0.02 %
4	H2O		solvent	90 %
5	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.76 ppm	internal	indirect	0.2514495
1H	water	protons	4.76 ppm	internal	direct	1.0
15N	water	protons	4.76 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.76 ppm	internal	indirect	0.2514495
1H	water	protons	4.76 ppm	internal	direct	1.0
15N	water	protons	4.76 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.76 ppm	internal	indirect	0.2514495
1H	water	protons	4.76 ppm	internal	direct	1.0
15N	water	protons	4.76 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.37 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.76 ppm	internal	indirect	0.2514495
1H	water	protons	4.76 ppm	internal	direct	1.0
15N	water	protons	4.76 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RSC, Strand ID: A Detail

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Release date

2013-01-02

Citation

Silk fibroin as an organic polymer for controlled drug delivery
Sato, M., Tochio, N., Watanabe, S., Kigawa, T., Aizawa, T.
J. Control. Release. (2006), 111, 219-227, PubMed 16458987 , DOI 10.1016/j.jconrel.2005.12.009 , Abstract

Related entities 1. THE BOMBYX MORI LYSOZYME, : 1 : 2 : 246 entities Detail

Experiments performed 2 experiments Detail