BMRB: 11472

Complex structure of WWE in RNF146 with ATP

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

WWE domain in RNF146 with ATP

Entity

1. WWE domain in RNF146 with ATP, entity 1 (polymer, Thiol state: all free), 110 monomers, 12546.85 Da Detail

PSSGSSGFLD KPTLLSPEEL KAASRGNGEY AWYYEGRNGW WQYDERTSRE LEDAFSKGKK NTEMLIAGFL YVADLENMVQ YRRNEHGRRR KIKRDIIDIP KKGVSGPSSG

2. ATP (non-polymer), 507.181 Da

Total weight

13054.03 Da

Max. entity weight

12546.85 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.7 %, Completeness: 91.3 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (1183 of 1296)	90.8 % (623 of 686)	91.9 % (455 of 495)	91.3 % (105 of 115)
Backbone	90.0 % (585 of 650)	89.0 % (202 of 227)	90.3 % (287 of 318)	91.4 % (96 of 105)
Sidechain	92.5 % (688 of 744)	91.7 % (421 of 459)	93.8 % (258 of 275)	90.0 % (9 of 10)
Aromatic	91.5 % (108 of 118)	91.5 % (54 of 59)	91.1 % (51 of 56)	100.0 % (3 of 3)
Methyl	97.6 % (80 of 82)	97.6 % (40 of 41)	97.6 % (40 of 41)

1. entity 1

PSSGSSGFLD KPTLLSPEEL KAASRGNGEY AWYYEGRNGW WQYDERTSRE LEDAFSKGKK NTEMLIAGFL YVADLENMVQ YRRNEHGRRR KIKRDIIDIP KKGVSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0mM 13C, 15N-labeled protein; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3;

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	protein	1.0 mM
2	H2O		solvent	90 %
3	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RSF, Strand ID: A Detail

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Release date

2013-03-03

Citation

Complex structure of WWE domain in RNF146 with ATP
He, F., Inoue, M., Kigawa, T., Inoue, M., Terada, T., Muto, Y., Yokoyama, S.
FEBS Lett.

Related entities 1. WWE domain in RNF146 with ATP, entity 1, : 1 : 1 : 20 entities Detail

Interaction partners 1. WWE domain in RNF146 with ATP, entity 1, : 10 interactors Detail

Experiments performed 2 experiments Detail