BMRB: 11474

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 36 monomers, 4083.609 Da Detail

GSSGSSGKIF TCEYCNKVFK FKHSLQAHLR IHTNEK

2. ZINC ION (non-polymer), 65.409 Da

Total weight

4149.018 Da

Max. entity weight

4083.609 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.1 %, Completeness: 89.8 %, Completeness (bb): 83.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (386 of 430)	90.7 % (204 of 225)	90.4 % (150 of 166)	82.1 % (32 of 39)
Backbone	83.8 % (181 of 216)	82.7 % (62 of 75)	85.7 % (90 of 105)	80.6 % (29 of 36)
Sidechain	94.7 % (234 of 247)	94.7 % (142 of 150)	94.7 % (89 of 94)	100.0 % (3 of 3)
Aromatic	96.0 % (48 of 50)	96.0 % (24 of 25)	96.0 % (24 of 25)
Methyl	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)

1. protein

GSSGSSGKIF TCEYCNKVFK FKHSLQAHLR IHTNEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.08 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.35 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.35 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RUT, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 3 : 6 : 970 entities Detail

Interaction partners 1. protein, : 2 interactors Detail

Experiments performed 2 experiments Detail